Research Technician Skilled In Mouse Work

Axle; Rockville (MD); Presencial; USD 80 000 - 85 000; Tempo integral

Vantagens oferecidas por esta oferta de emprego

Paid Time Off and Holidays

401K match up to 5%

Educational Benefits for Career Growth

Employee Referral Bonus

Healthcare FSAs (FSA)

Parking Reimbursement Account (PRK)

Dependent Care Assistant Program (DCAP

Transportation Reimbursement Account (

Resumo da oferta

Axle, a bioscience and information technology company, seeks a Research Technician Skilled In Mouse Work to join NHGRI at NIH in Bethesda, MD. The role focuses on supporting NHGRI scientists with a range of cellular and molecular biology techniques.

Responsibilities include mouse breeding and tissue collection, qRT-PCR, Western blotting, immunohistochemistry, and coordination with FSASD researchers. Strong biosafety and biology background required.

Qualificações

• BSL1 biosafety level required.
• Degree in Biology or related field.
• Proficiency with MS Office and data analysis tools.
• Experience with mouse handling and tissue processing.
• Experience in manuscript writing and data presentation.
• Knowledge of Western blotting and cellular assays.

Responsabilidades

• Plan, execute, and document laboratory experiments and procedures.
• Support NHGRI scientists with experimental development and protocol implementation.
• Coordinate with FSASD researchers on communications and planning.
• Perform cell culture, PCR/qRT-PCR, DNA/RNA assays, cloning, and protein assays.
• Operate laboratory instruments including microscopes and plate readers.
• Prepare specimens and maintain lab equipment and inventory.
• Plan and conduct in vitro and in vivo studies, including Western blotting and immunohistochemistry.
• Perform tissue culture, cell separation, and qualitative/quantitative analyses.

Conhecimentos

BSL1

Animal handling (Mouse)

Manuscript writing

Data presentation

Tissue sectioning

Primary cell culture

Western blotting

PowerPoint

Excel

Word

R

Formação académica

Biology degree or related field

Ferramentas

PowerPoint

Excel

Word

R

Descrição da oferta de emprego

Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH).

Axle is seeking a Research Technician Skilled In Mouse Work to join our vibrant team at the National Institutes of Health (NIH) supporting the National Human Genome Research Institute (NHGRI) located in Bethesda, MD.

Benefits We Offer:

• Paid Time Off and Paid Holidays
• 401K match up to 5%
• Educational Benefits for Career Growth
• Employee Referral Bonus
• Flexible Spending Accounts:
  • Healthcare (FSA)
  • Parking Reimbursement Account (PRK)
  • Dependent Care Assistant Program (DCAP)
  • Transportation Reimbursement Account (TRN)

Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH).

Axle is seeking a Research Technician Skilled In Mouse Work to join our vibrant team at the National Institutes of Health (NIH) supporting the National Human Genome Research Institute (NHGRI) located in Bethesda, MD.

Benefits We Offer:

• 100% Medical, Dental & Vision Coverage for Employees
• Paid Time Off and Paid Holidays
• 401K match up to 5%
• Educational Benefits for Career Growth
• Employee Referral Bonus
• Flexible Spending Accounts:
  • Healthcare (FSA)
  • Parking Reimbursement Account (PRK)
  • Dependent Care Assistant Program (DCAP)
  • Transportation Reimbursement Account (TRN)

The primary objective of the position is to provide research support to NHGRI scientists by performing a variety of assignments involving the application of a full range of cell biology related laboratory methods, procedures and techniques associated with NIH biomedical research environment.

Responsibilities

• Plans, executes, and arranges laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.
• Collaboratively work with mouse models of FSASD and GNE in NHGRI animal facility, activities include mouse breeding, observation, tissue collection, testing of therapeutics and documentation of breeding/tissue collection logistics.
• Provide research support to NHGRI scientists with planning, developing and implementing laboratory experiments and protocols; assist in coordinating communication with the Research Consortium on Free Sialic Acid Storage Disorder (FSASD).
• Perform laboratory technologies pertaining to molecular biology, biochemistry, glycobiology and cell biology. Such techniques include sterile cell culture, PCR, qRT-PCR, DNA/RNA as-says, cloning, protein assays, glycobiology tools, lectin technologies, antibody techniques.
• Operate a variety of laboratory instruments, including but not limited to (confocal)-microscopes, spectrophotometer/plate reader, other imaging equipment.
• Operates laboratory machines and instruments; and prepares specimens and equipment for use.
• Work with staff on processing specimens for embedding according to protocols.
• Maintain inventory system and sample storage according to outlined guidelines.
• Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence/ immunohistochemistry.
• Perform routine experiments and tests, including tissue culture, cell separation, protein, and quantitative and qualitative analysis.
• Perform primary cell culture techniques and immunohistochemistry.

Qualifications

• Biosafety Level 1 (BSL 1)
• Degree in Biology or related field
• PowerPoint, Excel, Word, R
• Animal Handling: Mouse
• Manuscript writing
• Tissue Sectioning
• Data Presentation
• Primary cell culture
• Western Blotting

Salary Range: $80,000 USD - $85,000 USD