Research Scientist II

Seattle Children's; Seattle (WA); On-site; USD 45,000 - 68,000; Full time

Job summary

Seattle Children’s is seeking a Research Scientist Level II to provide leadership and hands-on execution of in vivo projects focusing on lung fibrosis and T cell immunity against influenza. The role involves mouse infection models, tissue processing, flow cytometry, and bioinformatics analysis, with mentorship to junior staff.

The ideal candidate has strong immunology background, experience in in vivo experiments, and the ability to train others while contributing to manuscripts and grant

Qualifications

• Bachelor’s Degree in Immunology, Cell and Molecular Biology or related scientific discipline; may require a Master’s Degree for certain lab specialties.
• Minimum of two (2) years’ experience in a related area/laboratory setting.

Responsibilities

• Plan and perform mouse infection experiments with respiratory and other viral pathogens, bleomycin administration (o.p. route) under ABSL-2 conditions
• Administer substances via multiple routes, including oropharyngeal, intranasal, intravenous (tail vein), and intraperitoneal
• Perform mouse dissections, extraction of various mucosal tissues, BAL, lymph nodes, lung perfusion, and lung function testing using Flexivent instrument
• Carry out enzymatic and mechanical tissue digestion to generate single-cell suspensions
• Plan and execute single-cell RNA sequencing experiments
• Design, run, and analyze multiparameter flow cytometry experiments, including panel design, compensation, data analysis (e.g., FlowJo, Prism); FACS cell sorting
• Ensure compliance with IACUC protocols, biosafety requirements, and institutional policies
• Keep detailed, organized online laboratory records and maintain reagent and sample inventories
• Present data at lab meetings and contribute to manuscripts and grant preparation
• Train and support other lab members

Skills

Immunology

Cell & Molecular Biology

In vivo experiments

Training & mentoring

Education

Bachelor’s Degree in Immunology/Cell & Molecular Biology

Master’s may be required for some specialties

Tools

Flow cytometry

FACS cell sorting

FlowJo

Prism

Bioinformatics

Job description

The Joag Lab studies tissue-resident memory CD8 T cells (Trm) which stand guard in our airways, lungs, and other mucosal tissues. We want to know how these sentinels change from childhood to adulthood, and how those changes shape our protection against respiratory infections. We are also turning T cells into tools: in fibrotic diseases, where scarred tissue shuts out antibodies and other therapies, we are exploring how T cells can open the door and help treatments reach their targets. The Joag lab was established in 2025, and this position offers the chance to shape lab trajectory and culture from the ground up.

Position Summary

We are seeking a Research Scientist Level II to provide scientific leadership and technical execution of in vivo projects in lung fibrosis and T cell immunity against influenza. The position will involve in vivo mouse experiments, including viral infection models, bleomycin administration, in vivo lung-function testing (Flexivent), tissue extraction and enzymatic processing, flow cytometric analysis, and bioinformatics analysis. This person will have substantial experience in Immunology, Cell and Molecular Biology or related field and will help train and support other lab members.

Responsibilities

• Plan and perform mouse infection experiments with respiratory and other viral pathogens, bleomycin administration (o.p. route) under ABSL-2 conditions
• Administer substances via multiple routes, including oropharyngeal, intranasal, intravenous (tail vein), and intraperitoneal
• Perform mouse dissections, extraction of various mucosal tissues, BAL, lymph nodes, lung perfusion, and lung function testing using Flexivent instrument
• Carry out enzymatic and mechanical tissue digestion to generate single-cell suspensions
• Plan and execute single-cell RNA sequencing experiments
• Design, run, and analyze multiparameter flow cytometry experiments, including panel design, compensation, data analysis (e.g., FlowJo, Prism); FACS cell sorting
• Ensure compliance with IACUC protocols, biosafety requirements, and institutional policies
• Keep detailed, organized online laboratory records and maintain reagent and sample inventories
• Present data at lab meetings and contribute to manuscripts and grant preparation
• Train and support other lab members

Required Education And Experience

Bachelor’s Degree in Immunology, Cell and Molecular Biology or related scientific discipline; may require a Master’s Degree for certain lab specialties.

Minimum of two (2) years’ experience in a related area/laboratory setting.

Required Credentials

N/A.

Preferred

• Masters or PhD in Immunology, Cell and Molecular Biology or related scientific discipline.
• Proficiency in multiparameter flow cytometry, cell sorting, and data analysis
• mouse handling, and substance administration via various routes. Must be able to perform these tasks independently
• ability to focus during long in vivo mouse experiments, tissue digests, flow cytometry and complex experimental planning
• strong commitment to excellence, resilience, and good communication
• Design experiments in collaboration with PI
• Background in immunology, cell and molecular biology, or related field
• Experience with ABSL-2 and BSL-2 work

Additional experience in one or more of the following areas is desirable:

• Transcriptomic and/or genomic data analysis using R, Python, or related tools
• Advanced immunofluorescence microscopy
• Computational image analysis using ImageJ/Fiji, Python, Imaris or related tools

Personal qualities

• Strong work ethic and commitment to rigorous, reproducible science
• Exceptional attention to detail during all aspects of experimental work: design, execution, analysis, and record keeping
• Highly organized, able to manage multiple time-sensitive experiments at once
• Excellent and regular written and verbal communication with PI and other lab members
• Professional, respectful, and collaborative with colleagues at all levels
• maintain shared work calendar with lab members

Compensation Range

$32.87 - $49.31 per hour

Salary Information

This compensation range was calculated based on full-time employment (2080 hours worked per calendar year). Offers are determined by multiple factors including equity, skills, experience, and expertise, and may vary within the range provided.

Disclaimer for Out of State Applicants

This compensation range is specific to Seattle, positions located outside of Seattle may be compensated differently depending on various factors

Benefits Information

Seattle Children’s offers a generous benefit package, including medical, dental, and vision plans, 403(b), life insurance, paid time off, tuition reimbursement, and more. Additional details on our benefits can be found on our website www.seattlechildrens.org/careers/benefits.

About Us

Hope. Care. Cure. These three simple words capture what we do at Seattle Children’s – to help every child live the healthiest and most fulfilling life possible. Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE

Our founding promise to the community is as valid today as it was over a century ago: we will care for all children in our region, regardless of the families’ ability to pay. Together, we deliver superior patient care, advance new discoveries and treatments through pediatric research, and serve as the pediatric and adolescent, academic medical center for Washington, Alaska, Montana and Idaho – the largest region of any children’s hospital in the country.

U.S. News & World Report consistently ranks Seattle Children’s among the nation’s best children’s hospitals. For more than a decade, Seattle Children’s has been nationally recognized in key specialty areas. We are honored to be one of the nation’s very best places to care for children and the top-ranked pediatric hospital in Washington and the Pacific Northwest.

As a Magnet designated institution, we recognize the importance of hiring and developing great talent to provide best-in-class care to the patients and families we serve. Our organizational DNA takes form in our core values: Compassion, Excellence, Integrity, Collaboration, Equity and Innovation. Whether it’s delivering frontline care to our patients in a kind and caring manner, practicing the highest standards of quality and safety, or being relentlessly curious as we work towards eradicating childhood diseases, these values are the fabric of our culture and community. The future starts here.

Our Commitment

Seattle Children’s welcomes people of all experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our patients and families. Our organization recruits, employs, trains, compensates, and promotes based on merit without regard to race, religion, color, national origin, gender (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, status as a protected veteran, status as an individual with a disability, or other applicable legally protected characteristics.

The people who work at Seattle Children’s are members of a community that seeks to respect and celebrate all the qualities that make each of us unique. Each of us is empowered to be ourselves.

Seattle Children’s is proud to be an Equal Opportunity Workplace and Aff…