Research Technician II

Seattle Children's; Seattle (WA); On-site; USD 33,000 - 49,000; Full time

Benefits offered by this job

Medical, dental & vision plans

403(b) retirement plan

Life insurance

Paid time off

Tuition reimbursement

Job summary

Seattle Children’s Research Institute is seeking a Research Technician II for the Joag Lab to support studies on tissue-resident memory CD8 T cells across mucosal tissues. The role offers opportunity to influence lab trajectory and establish practices from the ground up.

The role emphasizes 20% lab mgmt, 25% animal colony mgmt, and 55% experimental research including flow cytometry and in vivo/in vitro mouse work. Strong skills in sterile technique, genotyping, and data analysis are valued.

Qualifications

• Bachelor's degree in biology or related field and at least 1 year of lab/research experience.
• Experience with molecular biology and biochemistry techniques is preferred.
• Hands-on lab skills including sterile technique and data recording.

Responsibilities

• 20% Lab Management: oversee operations, ordering, organization, equipment maintenance, regulatory compliance and records.
• 25% Animal Colony Management: manage mouse colony including breeding, genotyping, weaning.
• 55% Experimental Research: design/run flow cytometry, perform in vitro/in vivo mouse experiments, analyze data.

Skills

Lab management

Animal colony management

Flow cytometry

Mouse handling

Sterile technique

Data analysis

Education

Bachelor's degree in biology, microbiology, biochemistry, immunology, molecular biology, or related field

Tools

Flow cytometer

PCR

Job description

Research Technician II

The Joag Lab studies tissue-resident memory CD8 T cells (Trm) which stand guard in our airways, lungs, and other mucosal tissues. We want to know how these sentinels change from childhood to adulthood, and how those changes shape our protection against respiratory infections. We are also turning T cells into tools: in fibrotic diseases, where scarred tissue shuts out antibodies and other therapies, we are exploring how T cells can open the door and help treatments reach their targets. The Joag lab was established in 2025, and this position offers the chance to shape lab trajectory and culture from the ground up.

General job responsibilities

• 20% Lab Management: oversee day-to-day operations including ordering, organization, supply management, equipment maintenance, and regulatory compliance to ensure a well-functioning lab. Maintain detailed, updated and accurate records.


• 25% Animal Colony Management – manage a small-mid sized mouse colony including breeding, genotyping, weaning


• 55% Experimental Research – design and run flow cytometry experiments. independently perform in vitro and in vivo mouse experiments. Analyze, visualize, and present data to lab. Perform tasks independently and as part of a team effort on larger projects.

Preferred Technical Qualifications

• Multiparameter flow cytometry expertise is a highly valued asset


• Handling mice, administration of substances to mice via various routes (i.p., intranasal, intra-tracheal route, tail vein), and managing mouse colony


• Sterile cell culture technique, making buffers, solutions, dilutions


• Detailed note-taking, protocol writing, and experimental planning


• Tissue harvesting and enzymatic digestion

Desired Experimental skills

• Injection of mice via RO., I.T., I.N., IP, IV routes


• PCR, agarose gel electrophoresis

In your response, please identify specific examples from your past work, school or other experience that clearly highlight these experiment skills and personality traits. Please only describe your own prior experience.

Required Education and Experience
Bachelors Degree in biology, microbiology, biochemistry, immunology, molecular biology, or related field.
At least one (1) year of experience in a laboratory/research setting and/or experience with molecular biology and biochemistry techniques.

Required Credentials
N/A.

Compensation Range

$23.73 - $35.59 per hour

Salary Information

This compensation range was calculated based on full-time employment (2080 hours worked per calendar year). Offers are determined by multiple factors including equity, skills, experience, and expertise, and may vary within the range provided.

Disclaimer for Out of State Applicants

This compensation range is specific to Seattle, positions located outside of Seattle may be compensated differently depending on various factors.

Benefits Information

Seattle Children’s offers a generous benefit package, including medical, dental, and vision plans, 403(b), life insurance, paid time off, tuition reimbursement, and more. Additional details on our benefits can be found on our website www.seattlechildrens.org/careers/benefits.

About Us

Hope. Care. Cure. These three simple words capture what we do at Seattle Children’s – to help every child live the healthiest and most fulfilling life possible. Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE

Our founding promise to the community is as valid today as it was over a century ago: we will care for all children in our region, regardless of the families’ ability to pay. Together, we deliver superior patient care, advance new discoveries and treatments through pediatric research, and serve as the pediatric and adolescent, academic medical center for Washington, Alaska, Montana and Idaho – the largest region of any children’s hospital in the country.
U.S. News & World Report consistently ranks Seattle Children’s among the nation’s best children’s hospitals. For more than a decade, Seattle Children’s has been nationally recognized in key specialty areas. We are honored to be one of the nation’s very best places to care for children and the top-ranked pediatric hospital in Washington and the Pacific Northwest.

As a Magnet designated institution, we recognize the importance of hiring and developing great talent to provide best-in-class care to the patients and families we serve. Our organizational DNA takes form in our core values: Compassion, Excellence, Integrity, Collaboration, Equity and Innovation. Whether it’s delivering frontline care to our patients in a kind and caring manner, practicing the highest standards of quality and safety, or being relentlessly curious as we work towards eradicating childhood diseases, these values are the fabric of our culture and community. The future starts here.

Our Commitment

Seattle Children’s welcomes people of all experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our patients and families. Our organization recruits, employs, trains, compensates, and promotes based on merit without regard to race, religion, color, national origin, gender (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, status as a protected veteran, status as an individual with a disability, or other applicable legally protected characteristics.

The people who work at Seattle Children’s are members of a community that seeks to respect and celebrate all the qualities that make each of us unique. Each of us is empowered to be ourselves.

Seattle Children’s is proud to be an Equal Opportunity Workplace and