Research Scientist - Hawse Lab

University of Georgia- FACS; Pittsburgh, Northern (Allegheny County, KY); Hybrid; USD 54,000 - 89,000; Full time

Job summary

The University of Pittsburgh, Department of Immunology, is seeking a Research Scientist to study signaling and T cell differentiation using in vitro and in vivo approaches. The role includes isolating naïve CD4 T cells from mouse lymph nodes and spleens, and culturing under Th17-polarizing conditions to probe autoimmune disease mechanisms.

The successful candidate will design experiments, prepare manuscripts and grant proposals, and mentor staff and students in a collaborative lab on the

Qualifications

• Ph.D. degree required with 5+ years of experience.
• Experience in primary T cell culture and in vivo models.
• Ability to design and execute in vitro and in vivo autoimmune studies.

Responsibilities

• Perform isolation and culture of naïve CD4 T cells from mouse lymph nodes and spleen.
• Design and develop in vitro and in vivo experiments using animal models of autoimmunity.

Skills

T cell culture

RT-PCR

Western blot

In vitro

In vivo

Animal models

Education

Ph.D.

Tools

RT-PCR equipment

Western blot apparatus

Job description

Research Scientist - Hawse Lab

The Department of Immunology conducts exceptional and impactful research that uncovers new mechanisms and pathways of the immune system to translate discoveries to improve human health by combating disease, enhancing vaccination, and controlling cancer and autoimmunity; to incorporate and develop cutting-edge technologies, including systems and computational approaches; and to train the next generation of immunologists in a collegial and dynamic research environment.

The University of Pittsburgh, Department of Immunology, is seeking a qualified Research Scientist, Ph.D. Required plus 5 years of work experience.

This position will focus on studying research projects concerning signaling and T cell differentiation. This position will conduct scientific research using a combination of both in vitro and in vivo methodologies to comprehensively investigate the underlying mechanisms involved in autoimmune disease development. The in vitro component of the study will focus on the isolation and culture of nave CD4 T cells obtained from the lymph nodes and spleens of C57BL/6J mice. These cells will be cultured under Th17-polarizing conditions to promote their differentiation into Th17 cells. Th17 cells are a specialized subset of CD4+ T helper cells that have been extensively implicated in the pathogenesis and progression of various autoimmune disorders, making them a critical target for this investigation.

This position requires research experience designing and developing of in vitro and in vivo experiments in animal models of autoimmunity diseases. This position should have the technical skills such as primary cell culture (T cells), RT-PCR, western blot, and will perform the collection, interpretation, discussion and presentation of scientific data.

This position will prepare manuscripts, research grant proposals, research articles and publications, and give presentations on their research findings. This position will train Research Technicians, Postdoctoral Associates, Graduate Student Researchers and Pitt undergrad students.

These tasks require a skilled scientist who can independently work with minimum consultation with the principal investigator.

Job Summary

Conducts independent scientific research projects from inception to presentation. Prepares and crafts various documentations of research results into manuscripts, proposals, grant applications, and presentations. Trains undergraduates and research technicians. Manages laboratory policies and coordinates project development in collaboration with other specialists.

Essential Functions

1. Perform cell isolation and culture of nave CD4 T cells obtained from the lymph nodes and spleens of C57BL/6J mice.
2. Must design and develop in vitro and in vivo experiments in animal models of autoimmunity diseases.

Physical Effort

Minimal

Assignment Category: Full-time regular

Job Classification: Staff.Research Scientist

Job Family: Research

Job Sub Family: Research Science

Campus: Pittsburgh

Minimum Education Level Required: Professional Doctorate Degree

Minimum Years of Experience Required: 5

Will this position accept substitution in lieu of education or experience: No

Additional details about Required Licensure/Certification: None

Work Schedule: Monday - Friday, 8:30 a.m. - 5:00 p.m.

Work Arrangement: On-Campus: Teams that work on campus, in an office, or in a lab.

Hiring Range: $53,617 to $89,361 annual.

Relocation_Offered: No

Visa Sponsorship Provided: Yes

Background Check: For position finalists, employment with the University will require successful completion of a background check

Child Protection Clearances: Not Applicable

Required Documents: Resume

Optional Documents: Cover Letter

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