Laboratory Research Technician IV

University of Pittsburgh; Pittsburgh (Allegheny County); On-site; USD 50,000 - 70,000; Full time

Job summary

The University of Pittsburgh is seeking a Laboratory Research Technician IV to join the School of Medicine in Pittsburgh, PA. This full-time position involves the design and execution of various laboratory techniques including the isolation and activation of T cells, as well as performing a range of assays and data analysis using advanced software tools.

Candidates must have a master’s degree in a relevant field and at least one year of laboratory experience. The role requires availability to work weekends and holidays, contributing to impactful medical research.

Qualifications

• Master's degree (or equivalent) in Biology, Biotechnology, or related field.
• 1 year experience in a laboratory setting.
• Ability to work weekends and holidays.

Responsibilities

• Design and perform isolation, activation, and expansion of T cells.
• Conduct immunofluorescence imaging and various assays.
• Analyze data using software like GraphPad Prism and FlowJo.

Skills

Cryopreservation

Cell-based assays

Immunofluorescence imaging

Flow cytometry

Data analysis using GraphPad Prism

Education

Master's Degree in Biology or related field

Tools

GraphPad Prism

FlowJo

Excel

Image-J

Job description

Job Description - Laboratory Research Technician IV (26003389)

Laboratory Research Technician IV

Job Summary

Laboratory research technician iv

The University of Pittsburgh School of Medicine seeks a Laboratory Research Technician IV to work in Pittsburgh, PA. Duties include designing/performing: (i) isolation, activation, and expansion of T cells (ii) cryopreservation, sub‑culturing, and revival of various cell lines and primary cells; (iii) cell‑based assays including cytotoxicity, cell viability, and cell proliferation assays; (iv) immunofluorescence imaging using confocal microscopy and single‑cell time‑lapse imaging; (v) isolation and handling of PBMCs from human whole blood; (vi) multicolor flow cytometry, SDS‑PAGE, western blot, multi‑mode reader‑based assays and various biochemical assays; and (vii) data analysis using GraphPad Prism, FlowJo, Excel and Image‑J.

Must have a master’s degree (or foreign equivalent degree) in Biology, Biotechnology, or a related field plus one (1) year of experience in a related position.

Must have experience working with: (i) Cryopreservation, sub‑culturing and revival of various cell lines, mammalian cell lines and primary cells; (ii) In‑vitro assays such as cytotoxicity, cell viability, cell proliferation; (iii) Live cell time‑lapse imaging for cell viability assays, spheroids, immune cell killing, co‑culture assays and image analysis; (iv) Isolation of and assays using Peripheral Blood Mononuclear Cells (PBMCs) from human blood; (v) isolation and expansion of naïve T cells, CD4 T cells, CD8 T cells, and regulatory T cells; (vi) Activation and expansion of T cells using Dynabeads and plate coated antigen; (vii) Screening of peptide pools, cytokine/chemokine profiling using Luminex, and direct, indirect, Quantikine, and sandwich ELISA assays; (viii) Seed, treat, fix, permeabilize, block and antibody staining/addition of cell lines and primary immune cells for multicolor flow cytometry of surface and intracellular markers using appropriate compensation and FMO controls for different multi‑point in‑vitro assays; (ix) Analysis of the flow cytometry data for receptor quantification and drug screening using Flow‑Jo Software; (x) Preparing cells for molecular biology techniques such as SDS‑PAGE, western blot, protein/antibody quantification using Bradford/BCA assay and other multi‑mode reader‑based assays, bacterial transformation and cloning; and (xi) data analysis using GraphPad Prism and Excel. Image analysis using Image‑J.

Must be able to work weekends and holidays.

The University of Pittsburgh is an equal opportunity employer / disability / veteran.

Assignment Category

Full‑time regular

Campus

Pittsburgh

Minimum Education Level Required

Master's Degree

Minimum Years of Experience Required

1

Hiring Range

TBD Based On Qualifications

Background Check

For position finalists, employment with the University will require successful completion of a background check

Child Protection Clearances

Not Applicable