Research Scientist/Engineer 2 (NE S UAW Research)

University of Washington; Seattle (WA); On-site; USD 69,000 - 74,000; Full time

Benefits offered by this job

Benefits information available on UW

Job summary

The University of Washington Department of Pharmacology seeks a Research Scientist to lead overexpression, purification, and structural characterization of voltage-gated sodium channels in complex with insecticides. You will explore molecular mechanisms of insecticide action and binding interactions, contributing to our understanding of selectivity and efficacy.

This role emphasizes independent and collaborative research, training of junior personnel, and advancing structural biology using

Qualifications

• Bachelor’s degree in biology or related field with two years of lab experience.
• Extensive experience with molecular biology techniques.
• Experience with recombinant protein expression and purification.

Responsibilities

• Perform molecular biology techniques, including PCR, DNA isolation, cloning, sequencing, and construct generation.
• Perform mammalian and insect cell culture, recombinant protein expression, protein purification, biochemistry, and ligand-binding assays.
• Perform cryo-EM experiments, grid preparation, data collection, image processing, and 3D structure determination.
• Design, execute, and troubleshoot experiments to achieve research objectives.
• Manage research projects of moderate scope and evaluate feasibility of approaches.
• Train laboratory personnel in experimental techniques and SOPs.

Skills

Molecular biology

Protein expression

Cryo-EM

Experimental design

Education

Bachelor’s degree in biology/biochemistry/molecular biology

Tools

FPLC systems

Cryo-EM

Job description

Job Description

The Department of Pharmacology at the Wisedchaisri’s Lab investigates the structure and mechanism of voltage-gated ion channels, their interactions with drugs and neurotoxins, and the structural basis of human channelopathies caused by disease-associated mutations. We combine structural biology approaches, including X-ray crystallography and cryogenic electron microscopy (cryo-EM), with biochemistry and electrophysiology to perform comprehensive structural and functional studies of ion channels. Our goal is to uncover fundamental mechanistic insights that advance our understanding of ion channel function in health and disease and enable the development of innovative therapeutic strategies.

Job Description

The Department of Pharmacology at the Wisedchaisri Lab investigates the structure and mechanism of voltage-gated ion channels, their interactions with drugs and neurotoxins, and the structural basis of human channelopathies caused by disease-associated mutations. We combine structural biology approaches, including X-ray crystallography and cryogenic electron microscopy (cryo-EM), with biochemistry and electrophysiology to perform comprehensive structural and functional studies of ion channels. Our goal is to uncover fundamental mechanistic insights that advance our understanding of ion channel function in health and disease and enable the development of innovative therapeutic strategies.

About This Opportunity

Report to Research Assistant Professor, this Research Scientist will conduct independent and collaborative research focused on the overexpression, purification, and structural characterization of insect and human voltage-gated sodium channels in complex with insecticides. The successful candidate will investigate the molecular mechanisms of insecticide action on sodium channels and characterize insecticide–sodium channel binding interactions. This position will contribute to advancing our understanding of the molecular basis of insecticide selectivity and efficacy.

Key Responsibilities

Laboratory Research (75%)

• Perform molecular biology techniques, including PCR, DNA isolation, cloning, sequencing, and construct generation.
• Perform mammalian and insect cell culture, recombinant protein expression, protein purification, protein biochemistry, and ligand-binding assays.
• Perform cryo-EM experiments, including grid preparation, grid screening, data collection, image processing, and three-dimensional structure determination.
• Design, execute, and troubleshoot experiments to achieve research objectives.
• Manage research projects of moderate scope and evaluate the feasibility of experimental approaches.
• Develop, optimize, and implement new experimental procedures and improve existing protocols.
• Prepare and maintain laboratory reagents, solutions, and research materials.
• Assist with laboratory organization, equipment maintenance, and implementation of laboratory best practices.
• Train laboratory personnel in experimental techniques and standard operating procedures.

Data Analysis and Scientific Communication (20%)

• Maintain detailed and accurate experimental records.
• Analyze, interpret, and present experimental data.
• Present research findings at laboratory and sponsor meetings.
• Prepare publication-quality figures, datasets, and written materials for manuscripts, grant applications, reports, and scientific presentations.

Training and Mentoring (5%)

• Provide guidance, training, and technical direction to Research Scientist/Engineers, postdoctoral scholars, students, and other research personnel engaged in laboratory and research activities.
• Mentor junior laboratory members in experimental design, technical skills, data analysis, and scientific problem-solving.

Required Qualifications

To be considered for this opportunity, your application must demonstrate that you meet both the minimum qualifications and additional qualifications listed below. Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration.

Minimum Qualifications

• Bachelor's degree in biology, biochemistry, molecular biology, or related field and two years of laboratory experience.
• Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration.

Additional Qualifications

• Extensive experience with molecular biology techniques.
• Extensive experience with recombinant protein expression in mammalian expression systems and protein purification using FPLC systems.
• Demonstrated expertise in high-resolution cryo-EM, including grid preparation, high-throughput data collection, and image processing.
• Applicants who do not meet these qualifications WILL NOT be forwarded to the Hiring Manager.

Preferred Qualifications

• Experience with membrane protein structural biology.
• Experience training and supporting laboratory personnel.

Compensation, Benefits And Position Details

Pay Range Minimum

$68,736.00 annual

Pay Range Maximum

$74,244.00 annual

Benefits

Other Compensation: For information about benefits for this position, visit https://www.washington.edu/jobs/benefits-for-uw-staff/

Shift

First Shift (United States of America)

Temporary or Regular?

This is a regular position

FTE (Full-Time Equivalent)

100.00%

Union/Bargaining Unit

UAW Research

About The UW

Working at the University of Washington provides a unique opportunity to change lives – on our campuses, in our state and around the world. UW employees bring their boundless energy, creative problem-solving skills and dedication to building stronger minds and a healthier world. In return, they enjoy outstanding benefits, opportunities for professional growth and the chance to work in an environment known for its diversity, intellectual excitement, artistic pursuits and natural beauty.

Our Commitment

The University of Washington is committed to fostering an inclusive, respectful and welcoming community for all. As an equal opportunity employer, the University considers applicants for employment without regard to race, color, creed, religion, national origin, citizenship, sex, pregnancy, age, marital status, sexual orientation, gender identity or expression, genetic information, disability, or veteran status consistent with UW Executive Order No. 81. To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 or [e-mail]. Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current employment and past employment. Disclosure is required under Washington state law.