Research Scientist, Cell Biology

Two River; Cambridge (MA); On-site; USD 120,000 - 190,000; Full time

Job summary

Two River is seeking a creative cell biologist to join the discovery research team in Cambridge, MA. You will design and execute primary immune cell-based assays to profile multi-specific biologics and generate high-quality data for AI-assisted discovery.

The role requires hands-on experience with T cells, PBMCs, flow cytometry and functional assays, plus collaboration with computational colleagues to translate design hypotheses into experiments.

Qualifications

• PhD in immunology or related field with 2+ years industry experience.
• Hands-on experience with primary human immune cells and flow cytometry.
• Experience designing and executing cell-based assays and ELN documentation.

Responsibilities

• Design and optimize cell-based assays to assess activity and potency of multi-specific biologics.
• Maintain primary human immune cell systems and relevant cell lines.
• Develop quantitative readouts: flow cytometry, cytokine profiling, imaging.
• Document experiments rigorously in ELN and share data with computational team.
• Collaborate with computational and engineering colleagues to test design hypotheses.
• Present data in team meetings and ensure safety standards.

Skills

Immunology

Cell biology

Flow cytometry

ELN documentation

Team collaboration

Education

PhD in immunology or related field

MS/BS with industry experience

Tools

Flow cytometer

Cytometry analysis software

High-content imaging

Job description

Reports to: Director, Discovery Research

About Our Company:

We are a stealth-mode biotechnology company developing next generation complex biologics, with an initial focus on high-impact targets in immunology. Our mission is to rapidly design and globally develop highly differentiated therapies for patients with life-threatening diseases.

Position Overview:

We are seeking a creative, motivated and solutions-oriented cell biologist to join the discovery research team and generate the experimental biology that powers an integrated, AI-assisted discovery engine. Working at the bench and in close partnership with our computational colleagues, this individual will design and execute cell-based assays that characterize multi-specific biologics against targets in autoimmune disease and immuno‑oncology, and will produce the high-quality, structured data that closes our "lab-in-a-loop" - the iterative cycle in which computational design informs experiments and experimental results refine the next round of design. The role suits a hands-on scientist with deep experience in primary immune cell biology and functional assay development who is eager to work at the interface of wet-lab biology and computation.

Key Responsibilities:

• Design, optimize and execute cell-based assays to characterize the activity, potency and selectivity of multi-specific biologics, including T-cell-redirected cytotoxicity and co-culture killing assays.
• Establish and maintain primary human immune cell systems (e.g., T cells, PBMCs) and relevant cell line models for functional and mechanistic studies.
• Develop quantitative readouts of immune activation and function—flow cytometry, cytokine profiling, high-content imaging—to define mechanism of action and support candidate selection.
• Generate structured, well-annotated datasets and maintain rigorous electronic lab notebook records so results flow cleanly back into computational models and decision‑making.
• Partner with computational and protein engineering colleagues to translate design hypotheses into testable experiments and to interpret results in the context of ongoing design cycles.
• Troubleshoot assays, implement new techniques, and contribute to a strong culture of experimental rigor and reproducibility.
• Present data and interpretations in team and cross‑functional research meetings.
• Maintain high standards of laboratory safety.

Essential Qualifications:

• PhD in immunology, cell biology or a related discipline with 2+ years of relevant experience, or an MS/BS with 5+ years of industry experience.
• Hands‑on expertise culturing and manipulating primary human immune cells and cell line models.
• Strong experience in flow cytometry and cell‑based functional assays (e.g., cytotoxicity, proliferation, activation, cytokine release).
• Demonstrated ability to develop and optimize assays independently and to execute with rigor and attention to detail.
• Excellent record‑keeping using an ELN and a collaborative, proactive approach to teamwork.

Ideal Qualifications:

• Experience characterizing multi‑specific biologics; familiarity with T-cell engagers (TCE) or other complex modalities is a plus.
• Experience with in vitro models of autoimmune disease and/or immuno‑oncology.
• Comfort working in a data‑rich, computationally integrated environment and enthusiasm for AI‑assisted discovery.
• Experience with high‑content imaging or high‑throughput assay formats.

Two River is an equal opportunity employer. We are committed to building a team with a range of backgrounds, experiences and perspectives, and we evaluate all qualified applicants without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability, status as a protected veteran, genetic information, or any other characteristic protected by applicable law. We provide reasonable accommodations to applicants and employees with disabilities.