Research Scientist

University of Georgia- FACS; Pittsburgh (Allegheny County); Hybrid; USD 90,000 - 120,000; Full time

Job summary

The University of Pittsburgh, Department of Neurology, seeks a Research Scientist for a project on Parkinson's disease mechanisms after exposure to environmental toxicants. The role involves designing and executing cell-based assays, data analysis, and assisting in grant preparation.

On-site work in Pittsburgh with on-the-job training in cell culture and in vivo handling. Responsibilities include live imaging, RT-qPCR, western blotting, and data presentation.

Qualifications

• Professional Doctorate Degree in a relevant field.
• Minimum of 5 years of research experience.
• Experience with in vivo rodent work is preferred.

Responsibilities

• Design and perform properly powered in vitro assays using various cell lines.
• Analyze and graph data, prepare figures, and assist in grant writing.
• Train undergraduates and research technicians; interact with lab members.
• Perform live imaging, RT-qPCR, and western blotting; maintain cell culture.

Skills

Cell culture

RT-qPCR

Western blotting

Live imaging

Molecular biology

Education

Professional Doctorate Degree

Tools

CRISPR-Cas9

Seahorse

Confocal microscopy

Job description

Research Scientist

Med-Neurology - Pennsylvania-Pittsburgh - (26005827)

The University of Pittsburgh, Department of Neurology, would like to hire a Research Scientist. We are seeking applications for an upcoming project investigating divergent and convergent mechanisms of Parkinson's disease following exposure to disease relevant environmental toxicants. The successful candidate will be expected to design and carry out properly powered in vitro assays using various cell lines under direct supervision of the PI. The candidate will also be expected to analyze / graph data and prepares data figures and assist in grant writing. Interact closely with all lab members. This candidate will receive on the job training though prior cell culture experience and invivo handling is preferred. Tasks the scientist will perform include, cell culture maintenance, live imaging microscopy, RTqPCR, and western blotting.

Job Summary

Conducts independent scientific research projects from inception to presentation. Prepares and crafts various documentations of research results into manuscripts, proposals, grant applications, and presentations. Trains undergraduates and research technicians. Manages laboratory policies and coordinates project development in collaboration with other specialists.

Essential Functions

RS performs assays and keeps apprised of technical innovations and implement new techniques when appropriate. RS analyzes and graphs data generated by themselves or others in the lab, prepares data figures and illustrations for presentations and publications, and presents data at lab meetings and conferences. RS also writes manuscripts for publication in peer-reviewed journals, writes scientific abstracts for conferences, and assists in grant proposal writing. RS reviews literature and keeps the laboratory reference database updated. RS interacts closely with the PI, supervises and trains technicians and undergraduate students in the lab, and communicates with collaborators. Knowledge of in vivo rodent work, molecular biology (generating new plasmids, cell lines, sh/siRNA, CRISPR Cas9 technologies), cell culture (cell line, maintenance and engineering), resonant scanning confocal microscopy, live imaging microscopy are essential. Experience preferred in mitochondrial specific assays (eg. Seahorse), RTqPCR, Western Blotting, in situ proximity ligation, development of plate reader assays.

Physical Effort

Must be able to get around a university setting.

Assignment Category: Full-time regular

Job Classification: Staff.Research Scientist

Job Family: Research

Job Sub Family: Research Science

Campus: Pittsburgh

Minimum Education Level Required: Professional Doctorate Degree

Minimum Years of Experience Required: 5

Will this position accept substitution in lieu of education or experience: No

Work Schedule: M-F, 8:30-5:00

Work Arrangement: On-Campus: Teams that work on campus, in an office, or in a lab.

Hiring Range: TBD Based Upon Qualifications

Relocation_Offered: No

Visa Sponsorship Provided: No

Background Check: For position finalists, employment with the University will require successful completion of a background check

Child Protection Clearances: Not Applicable

Required Documents: Resume, Cover Letter

Optional Documents: Not Applicable

PI287173454