Research Lab Technician Associate

University of Michigan; Ann Arbor (MI); Hybrid; USD 42,000 - 54,000; Full time

Benefits offered by this job

Excellent medical, dental and vision

2:1 retirement match

Job summary

The University of Michigan’s Division of Pulmonary & Critical Care Medicine is seeking a Research Assistant or Laboratory Specialist for a VA Medical Center-based lab. The role focuses on cell and molecular biology techniques within immunology and related fields.

You will conduct experiments, manage animal tissue work, perform flow cytometry, RT-PCR, ELISA and other assays, and maintain meticulous records while ordering and maintaining lab materials.

Qualifications

• Bachelor's degree in health or biological science; pre-PhD or pre-med applicants encouraged.
• 0-1 year experience; general understanding of molecular biology, protein chemistry, immunology, and cell biology.
• Familiarity with laboratory techniques and instrumentation: solutions, analytical balances, pH meters, PCR, gel electrophoresis, spectrophotometers, coulter counters, scintillation counters, centrifuges.
• Experience handling mice and cell culture (animal) preferred.
• Ability to work independently and with supervision.

Responsibilities

• Initiate and perform experimental efforts in immunology/cell biology.
• Infect animals (mice) with microbes and dissect organs/tissues.
• Isolate cell populations from mice and stain cells for flow cytometry; acquire data with FACS.
• Analyze leukocyte subsets using gating strategies and FloJo program.
• Isolate RNA for RT-PCR and perform routine bioassays (ELISA, RT-PCR, CFU).
• Maintain records in notebooks and computer; manage ordering of lab supplies.
• Ensure equipment is functioning; arrange repairs if needed.
• Prepare media, buffers, and solutions; supervise purchasing of supplies.

Skills

Molecular biology

Protein chemistry

Immunology

Cell biology

Education

Bachelor's degree in health or biological science

Tools

PCR

Gel electrophoresis

Flow cytometry

Spectrophotometry

Centrifuges

Job description

Michigan Medicine improves the health of patients, populations and communities through excellence in education, patient care, community service, research and technology development, and through leadership activities in Michigan, nationally and internationally.Our mission is guided by our Strategic Principles and has three critical components; patient care, education and research that together enhance our contribution to society.

Job Summary

The Division of Pulmonary & Critical Care Medicine is seeking a Research Assistant or Laboratory Specialist for an existing laboratory located at the VA Medical Center Research Building. The Researcher will participate in ongoing research in a cell and molecular biology laboratory. This will involve a wide variety of techniques from the field of immunology/cell biology.

Responsibilities*

Duties include, but are not limited to:

* Under the direction of the principal investigator, initiate and perform experimental efforts entailing the following components:

Infect animals (mice) with microbes

Dissect the organs and tissues of infected animals

Isolate cell populations from mice.

Stain the cells with antibodies for flow cytometry experiments; acquire the data using our FACS system,

With provided gating strategy perform the analysis of leukocyte subsets (FloJo-program)

Isolate RNA for RT-PCR

Perform routine bioassays (ELISA, RT-PCR, CFU).

Perform various stimulated cell culture conditions over protracted time periods.

If experimental problems develop notify the PI/other personnel and assist in troubleshooting.

Record and enter the acquired data in notebooks and computer (excel and powerpoint),

Ordering materials and supplies for the laboratory.

Assuring that all the equipment is in working order, if problems arise, assist in requesting repair services.

Prepare media, buffers, and solutions.

Generate accurate laboratory records, and supervise the purchasing and production of laboratory supplies

Prepare inventories of animals, chemicals and samples.

Other duties as assigned.

Required Qualifications*

Bachelors degree in health or biological science discipline. Pre-PhD or pre-medical school applicants are encouraged to apply.

0-1 year experience

General understanding and preferably some experience with research in molecular biology, protein chemistry, immunology, and cell biology.

General understanding of biological and biochemical principles and practices

General understanding and preferably some experience biochemical laboratory techniques and instrumentation, such as preparing solutions, using analytical balances, pH meters, PCR, gel electrophoresis, spectrophotometers, coulter counters, scintillation counters, and centrifuges.

General understanding and preferably some experience of handling mice without reservation to handling the animals on daily basis.

General understanding and preferably some experience with cell culture techniques (animal).

Ability to work independently on assigned projects and analyses.

Ability to work effectively with supervisors and peers.

Desired Qualifications*

Previous experience in studying molecular biology, protein chemistry, immunology, and cell biology, Quantitative PCR, ELISA, staining cells with fluorescent monoclonal antibodies and flow cytometry experience, cell culture techniques, ability to work independently on pre-designed experiments and the initial data analysis.

Why Join Michigan Medicine?

Michigan Medicine is one of the largest health care complexes in the world and has been the site of many groundbreaking medical and technological advancements since the opening of the U-M Medical School in 1850. Michigan Medicine is comprised of over 30,000employees and our vision is to attract, inspire, and develop outstanding people in medicine, sciences, and healthcare to become one of the world's most distinguished academic health systems. In some way, great or small, every person here helps to advance this world-class institution. Work at Michigan Medicine and become a victor for the greater good.

What Benefits can you Look Forward to?

• Excellent medical, dental and vision coverage effective on your very first day
• 2:1 Match on retirement savings

Modes of Work

Positions that are eligible for hybrid or mobile/remote work mode are at the discretion of the hiring department. Work agreements are reviewed annually at a minimum and are subject to change at any time, and for any reason, throughout the course of employment. Learn more about the work modes .

Work Schedule

Monday through Friday, 8 hour days, hours negotiable

Michigan Medicine conducts background screening and pre-employment drug testing on job candidates upon acceptance of a contingent job offer and may use a third party administrator to conduct background screenings.Background screenings are performed in compliance with the Fair Credit Report Act. Pre-employment drug testing applies to all selected candidates, including new or additional faculty and staff appointments, as well as transfers from other U-M campuses.

Application Deadline

Job openings are posted for a minimum of seven calendar days.The review and selection process maybegin as early as the eighth day after posting.Thisopening may be removed from posting boards and filled any time after the minimum posting period has ended.

U-M EEO Statement

The University of Michigan is an Equal Opportunity Employer. We are committed to providing an environment of mutual respect where equal employment opportunities are available to all applicants, including protected veterans and individuals with disabilities.