Process Development Associate I — PRISM Cell Culture

Broad Institute; Cambridge (MA); On-site; USD 60,000 - 80,000; Full time

Benefits offered by this job

Medical Insurance

Dental Insurance

Vision Insurance

401(k) Retirement Plan

Paid Holidays

Job summary

The Broad Institute is seeking a Process Development Associate I to support the PRISM team in large-scale tissue culture production and molecular biology operations. You will manage culture of up to 30 cell lines, maintain a 1,000-line cell bank, and execute plate setups for screening campaigns.

Ideal candidates hold a BS/BA in a biology-related field with 1+ year of experience, strong aseptic technique, and excellent communication.

Qualifications

• BS/BA in Biology, Biomedical Engineering or related field.
• 1+ year experience in a biological research environment; mammalian culture preferred.
• Experience with basic molecular biology techniques (PCR, DNA extraction).
• Excellent written and oral communication; strong teamwork.
• Detail-oriented with thorough record-keeping and documentation.

Responsibilities

• Manage cell culture for up to 30 cell lines to generate PRISM pools.
• Maintain cell bank of 1,000 lines, barcoding and freezer stocks, inventories.
• Execute large-scale cell plating for production screens.
• Perform molecular biology assays including PCR, DNA/RNA extraction, gel electrophoresis for mycoplasma testing and STR shipments.
• Carry out CTG viability assays as needed.
• Keep comprehensive lab records using electronic notebooks (ELabNext).
• Prepare and maintain stocks of reagents and supplies; manage inventories.
• Perform routine equipment maintenance and update SOPs accordingly.
• Assess quality of PRISM pools using internal data visualization tools.
• Participate in team meetings to share results and align with goals.

Skills

Cell culture

Aseptic technique

PCR

DNA extraction

STR testing

Lab documentation

ELabNext

Teamwork

Communication

Education

BS/BA in Biology or related

Tools

ELabNext

Job description

The Broad Institute is seeking a Process Development Associate I to support the PRISM team in large-scale tissue culture production and molecular biology operations. You will manage culture of up to 30 cell lines, maintain a 1,000-line cell bank, and execute plate setups for screening campaigns.

Ideal candidates hold a BS/BA in a biology-related field with 1+ year of experience, strong aseptic technique, and excellent communication.