PRISM Process Development Associate I — Cell Culture

Broad Institute of MIT and Harvard; Cambridge (MA); On-site; USD 50,000 - 70,000; Full time

Benefits offered by this job

Medical insurance

Dental insurance

Vision insurance

Life insurance

Disability insurance

401(k) retirement plan

Flexible spending accounts

Health savings account

13 paid holidays

Winter closure

Paid time off

Parental leave

Employee assistance program

Job summary

The Broad Institute of MIT and Harvard is seeking a Process Development Associate I to support the PRISM team within the Cancer Program. The role focuses on routine cell culture and molecular biology operations across multiple cell lines, maintaining a 1,000-cell line bank, and generating pools for large-scale screening.

Applicants should have a biology-related BS/BA and 1+ year of lab experience, with strong aseptic technique and meticulous documentation.

Qualifications

• BS/BA in Biology, Biomedical Engineering or related fields.
• 1+ year experience in a biological research environment; experience with mammalian cell culture and aseptic technique preferred.
• Experience with basic molecular biology techniques including PCR and DNA extraction.
• Excellent written and oral communication and ability to work in a team.
• Strong organizational, analytical skills and attention to detail.

Responsibilities

• Manage cell culture for up to 30 cell lines to generate PRISM pools.
• Maintain a cell bank of 1000 cell lines, including barcoding new cell lines, creating new freezer stocks, and updating inventories/databases.
• Execute large-scale cell plating for production screens.
• Conduct molecular biology protocols and QC assays on cell lines (PCR, DNA/RNA extraction, gel electrophoresis for mycoplasma testing, sequence submission, STR testing).
• Execute CTG cell viability assays as needed.
• Maintain lab records, experimental write-ups, and processing notes using an ELN (eLabNext).
• Prepare and maintain stocks of reagents, solutions, and lab supplies; manage inventories.
• Perform routine maintenance on lab equipment to ensure functionality.
• Maintain and update SOPs to reflect production protocol changes.
• Assess quality of PRISM cell pools using internal data visualization tool.
• Participate in team meetings to share results and plan projects.

Skills

Cell culture

Aseptic technique

Molecular biology

PCR

DNA extraction

RNA extraction

Lab documentation

Team collaboration

Attention to detail

Data analysis

Communication

Education

BS/BA in Biology or Biomedical Engineering

Job description

The Broad Institute of MIT and Harvard is seeking a Process Development Associate I to support the PRISM team within the Cancer Program. The role focuses on routine cell culture and molecular biology operations across multiple cell lines, maintaining a 1,000-cell line bank, and generating pools for large-scale screening.

Applicants should have a biology-related BS/BA and 1+ year of lab experience, with strong aseptic technique and meticulous documentation.