Postdoctoral Fellowship

Office of Intramural Training & Education; Bethesda (MD); On-site; USD 65,000 - 75,000; Full time

Job summary

The National Cancer Institute's Experimental Immunology Branch in Bethesda invites applications for a Postdoctoral Fellowship to investigate how proteostasis regulates hematopoietic stem and progenitor cell fate and leukemogenesis. The project leverages genomics, transcriptomics, and proteomics with state-of-the-art resources.

The successful candidate will perform CRISPR-based gene editing, utilize advanced genome/chromatin analysis, and collaborate across immunology, molecular biology, and

Qualifications

• Ph.D. in Immunology, Biochemistry, Molecular and Cell Biology, or related disciplines.
• Knowledge and experience in CRISPR-based gene editing is a plus.
• Experience with genomics, transcriptomics, and proteomics approaches is desired.

Responsibilities

• Elucidate transcriptional and epigenetic programs induced by proteotoxic stress in hematopoietic stem and progenitor cells.
• Use relevant human cells and novel animal models to perturb and track stress-induced signaling pathways in real-time.

Skills

Genomics

Proteomics

Immunology

Molecular biology

Stem cell biology

Education

Ph.D. in Immunology, Biochemistry, Molecular and Cell Biology, or related disciplines

Tools

CRISPR editing

ATAC-seq

ChIP-seq

Transcriptomics

Proteomics

Job description

Experimental Immunology Branch, National Cancer Institute, NIH, Bethesda, MD and surrounding area

Scientific focus area

Cancer Biology,Cell Biology,Stem Cell Biology,Immunology,Molecular Biology and Biochemistry

A Postdoctoral Fellowship position is immediately available in the laboratory of Dr. Stanley Adoro within the Experimental Immunology Branch, NCI, in Bethesda, Maryland.

The central goal of our research is to better understand mechanisms that regulate normal blood cell generation from hematopoietic stem and progenitor cells. In parallel, we seek to elucidate cellular programs that prevent hematopoietic cell dysfunction and malignancy. While it is now well-established that deregulation in the cellular machinery that maintain genome integrity disrupts normal hematopoiesis and promote blood cancers, much less is known about how regulators of protein quality and proteome homeostasis influence blood cell differentiation, function and the emergence of blood cancers. Applying state-of-the-art approaches in genomics, transcriptomics, and proteomics to relevant human samples and animal models, our work specifically investigates how disruptions to proteome homeostasis shape blood cell development and the pathogenesis of blood disorders, especially acute leukemias (PMID: 39789376; 40319015).

The research projects

The current project will elucidate the transcriptional and epigenetic programs induced by proteotoxic stressors in hematopoietic stem and progenitor cells to support pre-malignant hematopoietic stem and progenitor cells and enable myeloid leukemogenesis. These studies will be performed using relevant human cells and novel genetic animal models that enable us to perturb and track proteotoxic stress-induced signaling pathways in real-time. The successful applicant will have access to state-of-the-art resources in confocal microscopy, transcriptomics, proteomics, genomics, and flow cytometry available at the EIB and NCI/CCR campus in Bethesda and NCI-Frederick.

Training opportunities

Fellows are encouraged to take advantage of multiple in-person and virtual seminars, focused courses (e.g., training in bioinformatics and translational research) and to actively engage in mentoring and participate in a variety of community activities, including various NIH Interest Groups in Immunology, Myeloid Malignancy, Stem Cells, and Proteostasis. Training opportunities are augmented by collaborations with extramural and NIH-wide intramural investigators, including at the Experimental Immunology Branch , who perform research in basic immunology, signal transduction and molecular mechanisms of diseases.

We are seeking a highly motivated candidate with a Ph.D. in Immunology, Biochemistry, Molecular and Cell Biology, or related disciplines.

Knowledge and experience in CRISPR-based gene editing, high-resolution genome/chromatin analysis technologies, and stem cell biology is a plus.