Postdoctoral Associate- Deverman Lab

Broad Institute; Cambridge (MA); On-site; USD 60,000 - 75,000; Full time

Job summary

The Deverman lab at the Broad Institute of MIT and Harvard is seeking a postdoctoral researcher or research scientist to join a multidisciplinary effort focused on developing AAV vectors for targeted gene expression in CNS neuronal populations. The role includes project planning, bench work, reporting results, and contributing to manuscripts and presentations.

You will develop high-throughput selection methods for capsids, apply gene delivery in vivo and in vitro, perform standard molecular

Qualifications

• 0-1+ years of related experience.
• A PhD in molecular biology, neuroscience, or related discipline.
• Experience with molecular biology techniques.
• Familiarity with NGS, high-throughput screening, immunohistochemistry/in situ hybridization, and production of gene delivery vectors is desirable.
• Hands-on experience with stereotaxic injections.
• Bioinformatics and statistics are helpful but not required.
• Outstanding interpersonal and communication skills.
• Proven ability to work within a large cross-disciplinary collaboration in a fast-paced environment.
• Excellent organizational and time management skills.

Responsibilities

• Develop, refine and apply high-throughput selection and screening methods for capsids and gene expression systems.
• Apply gene delivery strategies in vivo and in vitro.
• Use molecular biology techniques for plasmid construction, PCR, qPCR, RNA/DNA extraction, and NGS.
• Produce, purify and evaluate AAV.
• Troubleshoot and participate in new technology evaluation and implementation.
• Document, compile, and analyze data.
• Present results at team meetings and contribute to manuscripts and reports.
• Supervise activities of one or more research associates.
• Collaborate closely to design, implement, and troubleshoot essential experiments.

Skills

Molecular biology techniques

Team collaboration

Scientific communication

Education

PhD in molecular biology or neuroscience

Tools

NGS

PCR

qPCR

In situ hybridization

AAV production

Job description

Description & Requirements

The Deverman lab at the Broad Institute of MIT & Harvard is looking for an exceptional and energetic postdoctoral researcher or research scientist to join a rapidly growing, multidisciplinary technology development research effort aimed at developing AAV vectors that enable targeted gene expression in a range of disease-relevant CNS neuronal populations.

We seek a candidate to join an exciting and funded project aimed at developing gene regulatory elements and custom AAV capsids that will enable modern genetically encoded tools to be specifically expressed within specific neuronal subclasses. This project is a collaboration between multiple laboratories at the Broad Institute. The role will include project planning, hands-on bench work, reporting results to leadership, collaborators, funding agencies, and contributing to scientific manuscripts and presentations. The successful candidate will be an employee of the Broad Institute and will have access to a wide array of internal career development opportunities and scientific talks throughout the wider Harvard/MIT research ecosystem.

CHARACTERISTIC DUTIES

• Develop, refine and apply novel high-throughput selection and screening methods to develop novel capsids and gene expression systems
• Apply gene delivery strategies to multiple in vivo and in vitro models
• Use standard molecular biology techniques for plasmid construction, PCR, qPCR, RNA and DNA extraction and detection, and next generation sequencing (NGS)
• Conduct and/or supervise the production, purification and evaluation of AAV
• Troubleshoot to resolve problems and participate in new technology evaluation, development and implementation
• Document, compile, and analyze experimental data
• Attend and present at team meetings to share results, plan projects and experiments
• Make significant contributions to scientific manuscripts, funding agency reports, and conference presentations
• Supervise the activities of one or more research associates
• Work closely with team members to design, implement and troubleshoot experiments critical to the group's collective success

QUALIFICATIONS

• 0-1+ years of related experience
• A PhD in a relevant discipline molecular or cell biology, neuroscience, or related disciplines
• Experience with molecular biology techniques
• Familiarity with NGS, high-throughput screening methods, immunohistochemistry/in situ hybridization, and production of gene delivery vectors is strongly desired
• Hands on experience with stereotaxic injections
• Knowledge of bioinformatics and statistics is helpful but not required
• Outstanding interpersonal and communication skills
• Demonstrated track record of, and continued interest in, working as part of a large cross-disciplinary collaboration in a fast-paced research environment
• Exceptional organizational and time management skills

The expected base pay range for this position as listed above is based on a 40 hour per week schedule. Broad provides pay ranges representing its reasonable and good faith estimate of what the organization reasonably expects to pay for a position at the time of posting. Actual compensation will vary based on factors including but not limited to, relevant skills, experience, education, qualifications, and other factors permissible by law.

At Broad, your base pay is just one part of a comprehensive total rewards package. From day one, this role offers a competitive benefits package including medical, dental, vision, life, and disability insurance; a 401(k) retirement plan; flexible spending and health savings accounts; at least 13 paid holidays; winter closure; paid time off; parental and family care leave; and an employee assistance program, among other Broad benefits.

The Broad Institute is an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, national origin, religion, age, color, sex, disability, protected veteran status, or any other characteristic protected by local, state, or federal laws, rules, or regulations.

Should you need a reasonable accommodation to complete the application or interview process, please contact [e-mail] for assistance.