Postdoc - Long-Read RNA Methods for Neurodegeneration

Office of Intramural Training & Education; Framingham (MA); Presencial; USD 60.000 - 76.000; Jornada completa

Descripción de la vacante

The Translational Neurogenetics Unit at the Center for Alzheimer’s Disease and Related Dementias (CARD), National Institute on Aging (NIA), NIH, Framingham, MA invites applications for a Postdoctoral Fellow to develop and optimize long-read RNA methods for neurodegeneration using human brain tissue.

You will lead wet-lab protocol development, including low-input and degraded tissue, and explore direct RNA sequencing strategies.

Formación

• PhD in molecular biology, genomics, biochemistry, neuroscience or a related field with <5 years postdoc experience.
• Hands-on RNA work and NGS library prep experience required; ability to troubleshoot lab protocols.
• Experience with long-read sequencing and direct RNA sequencing is a plus.
• Ideas for applying or optimizing protocols to degraded/postmortem tissue encouraged.

Responsabilidades

• Lead wet-lab method development for long-read RNA sequencing in human brain tissue.
• Optimize protocols for low-input and degraded tissue and scale through automation.
• Validate findings with computational analyses and link isoforms to protein changes.
• Collaborate with CARD proteomics, and with external collaborators; publish and present findings.

Conocimientos

RNA work

Library preparation

Lab protocols development

Data analysis (R/Python/CLI)

Oxford Nanopore / PacBio

Educación

PhD in molecular biology/genomics/biochemistry/neuroscience

Herramientas

Oxford Nanopore

PacBio

Lab automation

R

Python

Descripción del empleo

The Translational Neurogenetics Unit at the Center for Alzheimer’s Disease and Related Dementias (CARD), National Institute on Aging (NIA), NIH, Framingham, MA invites applications for a Postdoctoral Fellow to develop and optimize long-read RNA methods for neurodegeneration using human brain tissue.

You will lead wet-lab protocol development, including low-input and degraded tissue, and explore direct RNA sequencing strategies.