Post Doc - Open Rank

University of Massachusetts Medical School; Bloomingdale (MA); On-site; USD 60,000 - 75,000; Full time

Job summary

University of Massachusetts Medical School is seeking a highly motivated Postdoctoral Associate for a lab focused on CAR Treg–based therapies and immune responses to genome editing in gene therapy. The role involves designing CAR Tregs, running assays to evaluate editing outcomes, and assessing immune responses to AAV or LNP‑delivered therapeutics.

This opportunity sits at the intersection of immunotherapy and gene editing.

Qualifications

• Ph.D. in Basic Science, Health Science, or a related field.
• Experience in cell culture, basic molecular biology skills, immunology assays, gene editing and gene therapy is preferred.
• Experience with designing mouse experiments is desirable.

Responsibilities

• Collaborates with team members to develop and refine genome editing strategies for in vivo editing and immune modulation using CAR Tregs.
• Designs and executes experiments to evaluate the safety and efficacy of genome‑edited cells and mouse models and assesses efficacy of therapeutics.
• Analyzes experimental data and interprets results; communicates findings effectively through presentations and publications.
• Contributes to preparation of grant proposals and scientific publications.

Skills

Immunology

Genome editing

Cell culture

Molecular biology

Immunology assays

Gene therapy

Mouse experiments

Education

Ph.D. in Basic Science/Health Science

Tools

CRISPR-Cas design

Next‑gen sequencing

ddPCR

ELISPOT

ELISA

Job description

Additional Information

Keeler-Klunk lab in gene therapy center is seeking a highly motivated scientist with expertise in immunology or genome engineering, to join our cutting‑edge laboratory focused on developing potent CAR Treg based therapies for neurological and hepatic disorders and assessing immune response to gene editing based therapeutics delivered as AAV or LNPs. Postdoctoral Associate will be responsible for designing CAR Tregs for modulating immune responses to gene therapies for diseases affecting neurological and hepatic disorders. Postdoctoral associate will also run assays to assess editing outcomes and assess immune response to gene editing reagents. This position offers a unique opportunity exposed to cutting edge translational research intersection of immunotherapy and gene editing technologies with the potential to make a significant impact on patient outcomes.

Qualifications- The ideal candidate must have a Ph.D. in Basic Science, Health Science, or a related field with an interest in working in translational science. Experience in cell culture, basic molecular biology skills, immunology assays, gene editing and gene therapy is preferred. Experience with designing mouse experiments is desirable.

Job Duties

• Collaborates with team members to develop and refine genome editing strategies for in vivo editing and immune modulation using CAR Tregs.


• Designs and executes experiments to evaluate the safety and efficacy of genome‑edited cells and mouse models and assesses efficacy of therapeutics.


• Assesses the functionality of engineered cells and evaluates their therapeutic potential both in vitro and in vivo that includes designing CRISPR‑Cas targeting strategies, analyzes editing outcomes on a molecular level using next gen sequencing and ddPCR and conducts functional assays such as cytotoxicity assays, ELISPOT, ELISA and cytokine release assays.


• Generate mRNA and Lipid nanoparticles, lentiviruses and retroviruses for different indications.


• Performs histological analysis to perform phenotypic assessment of editing outcomes.


• Analyzes experimental data and interprets results.


• Applies statistical methods to analyze data from experiments, that includes next‑generation sequencing data, flow cytometry data, and functional assay results.


• Communicates findings effectively through presentations in lab meetings, national conferences and scientific publications


• Contributes to the preparation of grant proposals and scientific publications.

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