Native MS Scientist — Structural Proteomics & Complexes

Buck Institute for Research on Aging; Novato (CA); Sur place; USD 85 000 - 110 000; Plein temps

Résumé du poste

Buck Institute for Research on Aging in Novato, CA is seeking a hands-on mass spectrometry scientist or postdoctoral researcher to establish and operate a structural MS platform. You will lead method development for native MS, crosslinking MS, and intact-protein analyses, collaborating with biochemists, structural biologists, and computational scientists.

The role emphasizes instrument operation, data interpretation, and development of workflows with quality control and automation, enabling

Qualifications

• PhD in analytical chemistry, biochemistry, biophysics, structural biology or related discipline.
• Hands-on operation of a high-resolution mass spectrometer, including setup, performance assessment and troubleshooting.
• Substantial practical expertise in native MS, crosslinking MS, HDX-MS, or intact-protein/top-down MS.
• Experience linking sample prep, acquisition settings, data analysis and experimental controls to reliable conclusions.
• Strong scientific reasoning, careful documentation, and ability to work independently and collaboratively.

Responsabilités

• Establish, optimize, and run structural-MS workflows, including low-flow LC for bottom‑up proteomics and crosslinking, and a separate LC workflow for intact‑protein and native MS analysis.
• Develop native-MS and/or crosslinking-MS methods to characterize protein-complex composition, stoichiometry, interactions, and structural changes.
• Prepare and evaluate samples, including buffer exchange, desalting, crosslinking, digestion, and enrichment; optimize native SEC or buffer exchange while assessing sample integrity.
• Optimize ionization, transmission, precursor isolation, and fragmentation for heterogeneous proteins and assemblies; troubleshoot and coordinate vendor support.
• Establish QC standards, reproducible protocols, and moving between native and denaturing analyses for higher throughput.
• Analyze spectra with appropriate controls, assess crosslink identifications and proteoform/PTM assignments.
• Maintain experimental records and organize raw data, analysis files, and protocols.
• Collaborate on experimental design, train colleagues, and contribute to publications.

Connaissances

Mass spectrometry operation

Data analysis

Sample preparation

Native MS

Crosslinking MS

HDX-MS

Top-down MS

Formation

PhD in analytical chemistry

PhD in biochemistry

PhD in biophysics

PhD in structural biology

Outils

High-resolution mass spectrometer

Description du poste

Buck Institute for Research on Aging in Novato, CA is seeking a hands-on mass spectrometry scientist or postdoctoral researcher to establish and operate a structural MS platform. You will lead method development for native MS, crosslinking MS, and intact-protein analyses, collaborating with biochemists, structural biologists, and computational scientists.

The role emphasizes instrument operation, data interpretation, and development of workflows with quality control and automation, enabling