Research Technician III

University of Pittsburgh; Pittsburgh (Allegheny County); On-site; USD 48,000 - 64,000; Full time

Job summary

The University of Pittsburgh's Pulmonary, Allergy, Critical Care, and Sleep Medicine Division seeks a Research Technician III to perform independent laboratory work, execute experiments, and prepare specimens and cultures under PI supervision.

Responsibilities include in vivo murine experiments, sterile cell culture, Western blotting, PCR, DNA/RNA prep, flow cytometry, data logging, and contributing to publications and grant proposals.

Qualifications

• Bachelor's degree in a relevant science field.
• At least 2 years of laboratory experience.
• Experience with sterile tissue culture,Western blotting,PCR, and DNA/RNA preparation.
• Experience with in vivo murine experiments preferred.
• Strong data management and organizational skills.

Responsibilities

• Assist in in vivo murine experiments and tissue harvesting.
• Perform sterile cell culture, drug treatments, and DNA/RNA transfections.
• Conduct genetic knockdown and techniques such as confocal microscopy, Western blotting, and flow cytometry.
• Maintain buffers, tissue culture rooms, procurement, and mouse colonies.
• Accurately log data and prepare figures for publication.

Skills

Sterile cell culture

Western blotting

PCR

Flow cytometry

Data analysis

Education

Bachelor's Degree

Tools

Confocal microscopy

DNA/RNA extraction

PCR machine

Flow cytometer

Job description

Performs a variety of independent laboratory work and experiments such as determining experimental protocols and preparation of specimens and cultures. Prepares reports of findings subsequent to compilation and analyzation. Oversees specimen inventory. Develops documentation for publication; crafts manuscripts and grant proposals.

Med-Medicine

The Pulmonary, Allergy, Critical Care, and Sleep Medicine Division is made of up approximately 70 clinical staff and over 100 research staff who work in a variety of locations including: Bridgeside Point, Biomedical Science Tower (BST), UPMC Hospitals including Presbyterian, Shadyside, East, Jameson and Mercy. The division provides world class services across 10 inpatient services at five hospitals and over 70,000 outpatient office visits. We have a major research mission that focuses on basic, clinical, and translational research on the lung, microbiome, COVID-19, sleep medicine, and allergy and immunology.

Under the supervision of the Principal Investigator, the research technician is responsible for executing experiments within the Acute Lung Injury Center of Excellence focused on lung macrophage responses to bacterial infection. This position requires experience in basic biochemical/molecular techniques including sterile tissue culture technique, western blots, PCR, and DNA/ RNA preparation, and flow cytometry with training in more advanced techniques to be provided. Previous experience with experimental mouse models is preferred.

Essential Functions

Assist laboratory staff with in vivo murine experiments through management of treated mice, tissue harvesting for in vivo and ex vivo studies, and management of mouse colonies for experiments.

Perform sterile cell culture experiments, drug treatments, and DNA/RNA transfections in vitro.

Perform genetic knockdown and other specialty techniques as assigned including but not limited to confocal microscopy, western blotting, and flow cytometry.

Assist with laboratory maintenance with preparation of standard buffers, tissue culture room maintenance, order procurement, and mouse colony maintenance.

Maintain meticulous, accurate, and timely data and log books.

Analytical reasoning skills required for preparation of data, graphs, tables, and charts to document experimental results for publication.

Physical Effort

Must be able to sit or stand at bench for a few hours, able to carry and open boxes for lab reagents that arrive, able to pipette for experiments.

The University of Pittsburgh is an equal opportunity employer / disability / veteran.

Assignment Category

Full-time regular

Job Classification

Staff.Laboratory Research Technician III

Job Family

Research

Job Sub-Family

Laboratory

Campus

Pittsburgh

Minimum Education Level Required

Bachelor's Degree

Minimum Years Of Experience Required

2

Will this position accept substitution in lieu of education or experience?

Combination of education and relevant experience will be considered in lieu of education and/ or experience requirement.

Work Schedule

Monday - Friday; 9am - 5:30pm

Work Arrangement

On-Campus: Teams that work on campus, in an office, or in a lab.

Hiring Range

To Be Determined Based On Qualifications

Relocation Offered

No

Visa Sponsorship Provided

No

Background Check

For position finalists, employment with the University will require successful completion of a background check

Child Protection Clearances

Not Applicable

Required Documents

Resume

Optional Documents

Cover Letter