Junior or Assistant Specialist - Parent lab

University of California San Francisco; San Francisco (CA); On-site; USD 57,000 - 73,200; Full time

Job summary

UCSF Diabetes Center is seeking a Junior or Assistant Specialist at the Parent lab for full-time engagement. The role covers culturing human and pluripotent stem cells, genome engineering, and cell analysis using immunohistochemistry, qPCR, and flow cytometry.

Responsibilities include lab organization, ordering supplies, and executing standard testing with careful interpretation of results. The ideal candidate holds a BA/BS (Junior) or a Master’s (Assistant) in biological sciences and has

Qualifications

• BA/BS degree in biological or biochemical sciences (Junior rank).
• Master's degree in biological or biochemical sciences (Assistant rank).
• Experience with cell culture methodologies.

Responsibilities

• Culture of primary human cells and pluripotent stem cells.
• Genome engineering of cells using TALEN/CRISPR.
• Transfection and/or viral infection of mammalian cells.
• Analysis of cells by immunohistochemistry, qPCR and flow cytometry.
• Participate in lab organization tasks such as ordering and maintaining supplies.
• Design and execute laboratory testing, make observations and interpret findings.

Skills

BA/BS degree in biological/biochemical

MS degree in biological/biochemical

Cell culture experience

Education

BA/BS in biological/biochemical sciences

Master's in biological/biochemical sciences

Job description

Junior or Assistant Specialist - Parent lab

The Parent lab at the UCSF Diabetes Center is seeking applicants for a full-time Specialist position at the Junior or Assistant rank. Our research is focused on understanding changes in immune tolerance that lead to autoimmune destruction of pancreatic insulin-producing beta cells in type 1 diabetes (T1D). We are combining directed differentiation of human pluripotent stem cells into cell types relevant to T1D and/or organoid culture of primary cells with genome engineering approaches to model the human disease. We are also developing strategies to produce immune evasive stem cell-derived pancreatic beta cells to accelerate clinical translation of cell therapies to treat diabetes.

The work includes:

• Culture of primary human cells and pluripotent stem cells
• Genome engineering of cells using techniques like TALEN/CRISPR
• Transfection and/or viral infection of mammalian cells
• Analysis of cells by immunohistochemistry, qPCR and flow cytometry
• The candidate is also required to participate in lab organization tasks such as ordering and maintaining lab supplies and equipment
• The candidate will also design and execute laboratory testing according to standard procedures,make observations and interpret findings

Required Qualifications:

• Specialist appointed at the Junior rank must possess (or in process of obtaining) a BA/BS degree in biological or biochemical sciences (or a related field).
• Specialist appointed at the Assistant rank must possess (or in process of obtaining) a Master's degree in biological or biochemical sciences (or a related field).
• Applicants' materials must list (pending) qualifications upon submission.
• Applicants must have obtained the degree requirement for the Specialist rank bythetime of hire.
• Experience with cell culture methodologies.

Preferred Qualifications:

• Experience with confocal microscopy, spectral flow cytometry, immunostaining, single-cell preparation, and molecular biology workflows.
• Strong organization skills and the ability to work under minimal supervision are key to thisposition.

See Table 24B for the salary range for this position. A reasonable estimate for this position is $57,000 - $73,200.

• Specialist appointed at the Junior rank must possess (or in process of obtaining) a BA/BS degree in biological or biochemical sciences (or a related field).
• Specialist appointed at the Assistant rank must possess (or in process of obtaining) a Master's degree in biological or biochemical sciences (or a related field).
• Applicants' materials must list (pending) qualifications upon submission.