In Vivo Pharmacology Research Assistant

Clora; Cambridge (MA); On-site; USD 55,000 - 96,000; Full time

Job summary

Clora is seeking a Research Associate for a ~6-month, onsite role in Cambridge, MA. The position involves conducting in vivo pharmacology studies in rodent models, histology and imaging, and molecular biology to generate high-quality biodistribution and target engagement data.

The ideal candidate will have a bachelor’s degree in Cell Biology or related field, rodent surgical experience, hands-on histology, and experience with multiplex IHC/IF and image analysis.

Qualifications

• Bachelor’s degree in Cell Biology or related field with 1+ years of laboratory experience.
• Rodent surgical experience, specifically stereotactic/ICV drug delivery to mouse brain.
• Strong hands-on experience in histology, including embedding, sectioning, staining, IHC/IF, and imaging.
• Authorship on a peer reviewed publication.
• Experience with multiplex IHC/IF and advanced image analysis platforms.

Responsibilities

• In Vivo Pharmacology - conduct in vivo studies in rodent models with peripheral administration and CNS delivery methods.
• Conduct terminal procedures, including animal takedowns, blood collection, and necropsies.
• Perform tissue harvesting and dissection for downstream analyses.
• Histology & Imaging - prepare, embed, and section tissues using microtome, vibratome, or cryostat (FFPE or fresh-frozen).
• Perform IHC/IF on tissue sections and acquire high-quality images with fluorescence/confocal systems.
• Conduct in vivo and ex vivo fluorescence and bioluminescence imaging using small-animal imaging systems and analyze distribution.
• Perform quantitative image analysis using ImageJ, QuPath, Halo or equivalent.

Skills

In vivo pharmacology

Histology & imaging

Molecular biology

Data analysis

Scientific writing

Education

Bachelor’s degree in Cell Biology or related field

Tools

ImageJ

QuPath

Halo

IVIS

Western blot

qRT-PCR

Job description

*1099 Consultant Opportunity*

Onsite- 5 days per week in Cambridge, MA

40 hours per week for ~6 months

Clora's client is a venture-backed medical research organization with access to a wide portfolio of product technology and research platforms. We are seeking a highly motivated Research Associate with broad expertise conducting in vivo studies, histology preparation and analysis, and molecular biology. This individual will play an important role in generating high-quality data on the biodistribution, target engagement, and biological response of our molecules in animal models. The candidate must be independent, goal-oriented, have excellent communication skills, and be willing to work in a highly dynamic and fast-paced entrepreneurial environment. This is a unique opportunity to drive the growth of an early-stage biotechnology company where you can contribute to the next frontier of genetic medicines. We are looking for collaborative, enthusiastic problem-solvers to join our team, driven by a shared goal of making a practical impact.

Responsibilities:

• In Vivo Pharmacology - Conduct in vivo studies in rodent models including experience with peripheral administration (IV, IP, SC) and stereotactic surgeries for direct to CNS administration
• Conduct terminal procedures, including animal takedowns, blood collection, and necropsies
• Perform tissue harvesting and dissection as appropriate for downstream bioanalytical and histological applications.
• Histology & Imaging - Prepare, embed, and section a variety of mammalian tissues using microtome, vibratome, or cryostat techniques (FFPE or fresh-frozen samples)
• Perform immunohistochemistry (IHC) and immunofluorescence (IF) on tissue sections
• Acquire high-quality images using epifluorescence and/or confocal microscopy
• Perform in vivo and ex vivo fluorescence and bioluminescence imaging using IVIS or equivalent small-animal imaging systems, including organ collection, image acquisition, and quantitative analysis of signal intensity and tissue distribution
• Conduct quantitative image analysis (cell counts, regional MFI, colocalization analysis, morphometric quantification) using software such as ImageJ, QuPath, Halo, or equivalent platforms.
• Molecular Biology - Perform measurement of gene and protein expression analyses using qRT-PCR, Western blotting, and ELISA methodologies and interpret data. Report results clearly to the scientific team.

Ideal Candidate:

• Bachelor’s degree or higher in Cell Biology or a related field, with 1+ years of laboratory experience, preferably in the biopharmaceutical industry.
• Rodent surgical experience, specifically stereotactic/ICV drug delivery to mouse brain
• Strong hands-on experience in histology, including tissue embedding, sectioning, staining, IHC/IF, and imaging.
• Authorship on a peer reviewed publication.
• Experience with multiplex IHC/IF and advanced image analysis platforms.