Immunology Research Assistant: Primary Cells & Gene Expression

University of Florida; Gainesville (FL); On-site; USD 40,000 - 52,000; Full time

Job summary

The Griffin Lab at the University of Florida seeks a Research Assistant I to support studies on nuclear receptor signaling and innate/adaptive immune responses. You will isolate and culture primary cells, conduct immune stimulation assays, and assist with gene expression analyses.

Required: bachelor's degree with lab experience; preferred MS in immunology or related field; experience with RT-qPCR, RNA-seq prep, and flow cytometry. Full-time, on-site role with veteran's preference eligibility.

Qualifications

• Hands-on experience with primary cell culture and aseptic technique.
• Experience with RT-qPCR and basic molecular biology methods including plasmid transfection.
• Strong organizational skills and attention to detail.

Responsibilities

• Isolate, culture, and maintain primary cells.
• Perform immune stimulation assays.
• Quantify gene expression by RT-qPCR; assist with RNA-seq and CUT&RUN prep.
• Maintain cell lines and perform routine molecular biology (RNA/DNA extraction, cloning, western blot).
• Maintain detailed lab notebooks and assist with data analysis/ figure preparation.
• Manage lab inventory, reagent ordering, and equipment maintenance.
• Assist with IACUC-related animal procedures (if applicable).

Skills

Primary cell culture

Aseptic technique

Strong organizational skills

Education

Bachelor's degree + 2 years experience

Tools

RT-qPCR

RNA/DNA extraction

Cloning

Western blot

RNA-seq prep

Job description

The Griffin Lab at the University of Florida seeks a Research Assistant I to support studies on nuclear receptor signaling and innate/adaptive immune responses. You will isolate and culture primary cells, conduct immune stimulation assays, and assist with gene expression analyses.

Required: bachelor's degree with lab experience; preferred MS in immunology or related field; experience with RT-qPCR, RNA-seq prep, and flow cytometry. Full-time, on-site role with veteran's preference eligibility.