Experienced Scientist, Proteomics

6084-Janssen Research & Development, LLC Legal Entity; Cambridge (MA); On-site; USD 94,000 - 152,000; Full time

Benefits offered by this job

Pension plan

401(k)

Job summary

Johnson & Johnson in Cambridge, MA is seeking an Experienced Scientist, Proteomics to join the Discovery Proteomic Platforms group. You will apply cutting-edge mass spectrometry to high-scale analyses and collaborate across multiomics teams.

This hands-on role involves bench work, protein analytics, and clear scientific communication as you work with senior scientists to drive end-to-end proteomics studies from sample prep to data interpretation.

Qualifications

• Master's degree with 1+ years or Bachelor's degree with 3+ years in a proteomics lab setting
• Minimum of 2 years of experience with mammalian cell culture
• Experience with label-free proteomics workflows and robust sample preparation techniques
• Strong planning and organizational skills with the ability to handle several projects simultaneously

Responsibilities

• Joining a team of wet lab scientists enabling proteomic impact across programs
• Planning and performing wet lab proteomic activities supporting evaluation of therapeutic candidates
• Generating samples for proteomics analysis from cell lines, tissues, plasma/serum, etc.
• Maintaining cell lines in flasks and plates
• Preparing assay plates and performing chemical treatments and perturbations
• Operating LC-MS instrumentation and maintaining associated systems

Skills

Mammalian cell culture

Label-free proteomics

Planning and organizational skills

Interpersonal skills

Education

Master's or Bachelor's with proteomics lab experience

Tools

Proteome Discoverer

MaxQuant

DIA-NN

Spectronaut

Skyline

Thermo Orbitrap Exploris

Job description

At Johnson & Johnson, we believe health is everything. Our strength in healthcare innovation empowers us to build a world where complex diseases are prevented, treated, and cured, where treatments are smarter and less invasive, and solutions are personal. Through our expertise in Innovative Medicine and MedTech, we are uniquely positioned to innovate across the full spectrum of healthcare solutions today to deliver the breakthroughs of tomorrow, and profoundly impact health for humanity. Learn more at jnj.com

Job Function

Discovery & Pre-Clinical/Clinical Development

Job Sub Function

Biological Research

Job Category

Scientific/Technology

All Job Posting Locations

Cambridge, Massachusetts, United States of America

Job Description

About Innovative Medicine Our expertise in Innovative Medicine is informed and inspired by patients, whose insights fuel our science-based advancements. Visionaries like you work on teams that save lives by developing the medicines of tomorrow. Join us in developing treatments, finding cures, and pioneering the path from lab to life while championing patients every step of the way. Learn more at https://www.jnj.com/innovative-medicine

We are searching for the best talent for an Experienced Scientist, Proteomics to be located in Cambridge, MA.

Purpose

Do you want to use your hard-earned skills and drive to make a difference in the lives of patients? Join the growing Discovery Proteomic Platforms (DPP) group within the Discovery Technologies and Molecular Pharmacology (DTMP) department of Therapeutics Discovery to apply groundbreaking approaches to high-scale proteomic analysis. Be part of a motivated team deploying cutting-edge mass spectrometry technologies to tackle challenges in end-to-end drug discovery and development. Work across a leading multiomics organization to provide coordinated insights across a variety of 'omics layers. This is a hands-on laboratory role, fit for a scientist who thrives at the bench and wants to grow in experimental design and scientific communication by working closely with senior scientists. With your help, we will drive development of medicines for our patients!

Responsibilities

• Joining a team of wet lab scientists enabling proteomic impact across a wide variety of programs and therapeutic modalities.
• Planning and performing wet lab proteomic activities supporting evaluation of therapeutic candidates.
• Generating samples for proteomics analysis from multiple sources (immortalized cell lines, FFPE, fresh-frozen tissue, plasma/serum, etc.).
• Maintaining multiple cell lines in both flasks and plates.
• Preparing assay plates and performing chemical treatments and perturbations.
• Applying high-efficiency proteomics sample preparation methodologies across many experiment types (global proteomic profiling, immunoprecipitation, affinity, PTM analysis).
• Operating and maintaining LC-MS instrumentation (Thermo Orbitrap Exploris/Eclipse/Astral, Bruker timsTOF; LC systems such as Vanquish Neo, Evosep One, UltiMate 3000), including daily/weekly maintenance, calibration, and troubleshooting.
• Deploying automation-supported workflows for high-throughput sample prep using platforms such as Hamilton Microlab STAR, Tecan Fluent, AssayMAP Bravo, Beckman Biomek, or Dynamic Devices Lynx.
• Ensuring QC and reproducibility, including system suitability checks, performance tracking, and SOP documentation.
• Collaborating with colleagues within the proteomics group to drive proteomics experiments across the drug discovery portfolio.
• Demonstrating creativity and applying novel approaches to solving scientific problems.
• Proposing novel and practical ideas aligned with scientific strategies and demands.

Qualifications / Requirements

Education

A Master's degree with 1+ years, or a Bachelor's degree with 3+ years, of proven experience in a proteomics laboratory setting (in Analytical Chemistry, Biochemistry, Chemical Biology, Molecular Biology, Pharmacology, or a related field).

Experience and Skills

Required

• Minimum of 2 years of experience with mammalian cell culture and treatment.
• Experience with label-free proteomics workflows and robust sample preparation techniques.
• Strong planning and organizational skills with the ability to handle several projects at a time in a fast-paced environment.
• Excellent interpersonal skills working with diverse colleagues.

Preferred

• Experience with liquid chromatography (Thermo Vanquish Neo, EASY-nLC, UltiMate 3000, or Evosep One) and mass spectrometry (Thermo Exploris, Eclipse, Lumos, Astral, or Bruker timsTOF) instrumentation.
• Experience with data processing suites such as Proteome Discoverer, MaxQuant, DIA-NN, Spectronaut, or Skyline.

Johnson & Johnson is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, disability, protected veteran status or other characteristics protected by federal, state or local law. We actively seek qualified candidates who are protected veterans and individuals with disabilities as defined under VEVRAA and Section 503 of the Rehabilitation Act. Johnson & Johnson is committed to providing an interview process that is inclusive of our applicants’ needs.

Salary & Benefits

• The anticipated base pay range for this position is : $94,000.00 - $151,800.00
• Additional Description for Pay Transparency: ubject to the terms of their respective plans, employees are eligible to participate in the Company’s consolidated retirement plan (pension) and savings plan (401(k)). This position is eligible to participate in the Company’s long-term incentive program. Subject to the terms of their respective policies and date of hire, employees are eligible for the following time off benefits:
• Vacation –120 hours per calendar year
• Sick time - 40 hours per calendar year; for employees who reside in the State of Colorado –48 hours per calendar year; for employees who reside in the State of Washington –56 hours per calendar year
• Holiday pay, including Floating Holidays –13 days per calendar year
• Work, Personal and Family Time - up to 40 hours per calendar year
• Parental Leave – 480 hours within one year of the birth/adoption/foster care of a child
• Bereavement Leave – 240 hours for an immediate family member: 40 hours for an extended family member per calendar year
• Caregiver Leave – 80 hours in a 52-week rolling period
• 10 days Volunteer Leave – 32 hours per calendar year
• Military Spouse Time-Off – 80 hours per calendar year

At Johnson & Johnson, we believe health is everything. Our strength in healthcare innovation empowers us to build a world where complex diseases are prevented, treated, and cured, where treatments are smarter and less invasive, and solutions are personal. Through our expertise in Innovative Medicine and MedTech, we are uniquely positioned to innovate across the full spectrum of healthcare solutions today to deliver the breakthroughs of tomorrow, and profoundly impact health for humanity. Learn more at https://www.jnj.com/. Our Credo calls on us to respect the dignity of every individual and create an environment for all people to thrive. Throughout the recruitment process - from application through hiring - we are committed to providing reasonable accommodations/adjustments for qualified candidates with disabilities. If you need support at any stage of the recruitment process, please complete our candidate accommodation request form so we can assist you.