Core Research Facility Technician III - Electron Microscopy

CHEManager International; Gainesville (FL); On-site; USD 45,000 - 46,000; Full time

Job summary

CHEManager International is seeking a Core Research Facility Technician III for the Main Campus in Gainesville, FL. The role involves preparing specimens for TEM/SEM, training investigators, and maintaining lab records and scheduling.

Responsibilities include ultramicrotomy, cryo-EM sample prep, and supervising the lab in the absence of the Lab Manager. A BA/BS in a related field is required, with CEMT preferred or equivalent experience.

Qualifications

• BA/BS in related scientific field or equivalent combination of education and experience.
• Core or technology-specific licensure and/or certifications where applicable; annual training.
• Preference for CEMT certification or equivalent EM lab experience.

Responsibilities

• Prepare specimens for electron microscopy including tissue and microbes.
• Train investigators in TEM/SEM operation and related equipment.
• Maintain records, scheduling, and billing in ILab system.
• Perform ultramicrotomy and knife maintenance.
• Execute advanced techniques such as immunoelectron microscopy and cryo-EM data prep.
• Open/Close and supervise lab in absence of Lab Manager/Director.
• Collaborate with investigators to adapt techniques and interpret results.
• Maintain general microscope and equipment function.

Skills

Record-keeping

Communication

Team player

Education

BA/BS in related scientific field

Tools

Transmission electron microscope (TEM)

Scanning electron microscope (SEM)

Cryo-EM sample prep

Job description

Job no: 539359

Work type: Staff Full-Time

Location: Main Campus (Gainesville, FL)

Categories: Facilities Operations/Skilled Trades, Physical/Mathematical Sciences

Department: 11600104 - RE-ICBR-ELECTRON-MICROSCOPY

Classification Title

Core Research Facility Technician III

Job Description

• Prepare research specimens, including but not limited to animal and plant tissue and microbes, for examination by transmission and scanning electron microscopy, using a wide range of technical procedures.
• Train research investigators on the proper use of transmission and scanning electron microscopes and ancillary equipment.
• Maintain records of all specimens processed, including a detailed account of all steps used in processing each specimen. Maintain online scheduling, project tracking, and billing within the ILab system.
• Perform ultramicrotomy with both glass and diamond knives. Makes glass knives when required and maintains diamond knives.
• Perform advanced techniques such as immunoelectron microscopy, single particle cryo-TEM, pre-processing of data movies, and prepare samples and collect data for cryo-SEM.
• Open/Close and supervise the lab in the absence of the Lab Manager or Director.
• Work with research investigators in developing or modifying techniques appropriate to the electron microscopic study of their particular specimen and help them understand the results.
• Perform general maintenance (i.e., replace electron gun filament) and troubleshoot basic problems of electron microscopes and ancillary equipment.
• Other duties as assigned

Expected Salary

$45,000 - $46,000, commensurate with education and experience

Minimum Requirements

BA/BS in a related scientific field, or an equivalent combination of education and experience.

Core or technology-specific licensure and/or certifications, where applicable. Additionally, yearly certificate and compliance training, if required.

Preferred Qualifications

• Certified Electron Microscopy Technologist (CEMT) certification from the Microscopy Society of America and an A.A. degree or certification from a two-year EM program, or a minimum of 4 years of experience in a biological electron microscopy laboratory. If the applicant is not a CEMT, the new hire may be required to complete and pass the CEMT program.

• The ICBR EM lab is a fee-for-service facility where customer service and project tracking are an integral part of daily activities. Must have good record-keeping skills and communicate effectively with customers and other laboratory staff. A comprehensive knowledge and ability to perform principles and techniques used in electron microscopic study of biological specimens, to include: specimen preparation, ultramicrotomy, operation of transmission and scanning electron microscopes, operation of confocal microscope, and the recording of images. Should have a basic knowledge of cell and tissue ultrastructure. Ability to work independently is essential, and yet, important to be a team player. Trains others in techniques and instrumentation. Desire to learn advanced techniques and new methodologies.