Biologist (Electron Microscopy)

GAP Solutions Inc; Baltimore (MD); On-site; USD 70,000 - 110,000; Full time

Job summary

GAP Solutions Inc in Maryland seeks a Biologist to support the NIA confocal imaging facility. You will perform wet-lab work, microscopy, and data analysis to meet NIH objectives.

Responsibilities include confocal image acquisition, IHC/IF, TEM sample prep, and 3D reconstruction, plus training staff and maintaining equipment and records. A Master's in Biology and 4+ years' bio lab experience are required.

Qualifications

• Master's degree in biology or related field.
• Experience with Confocal Light Microscopy and image analysis.
• 4 years in a biological research setting with embedding and TEM prep.
• 2–5 years of SEM/TEM experience.
• Familiarity with 3D reconstruction software.

Responsibilities

• Perform Confocal imaging and analysis for the NIA Confocal Imaging Facility.
• Prepare TEM samples and immunohistochemistry/immunofluorescence workflows.
• Operate TEM and related support equipment; troubleshoot lab protocols.
• Train staff and support researchers in confocal microscopy and data management.
• Maintain lab records, supplies, and equipment; ensure clean, organized workspace.

Skills

Confocal Light Microscopy

3D Reconstruction Software

Strong communication skills

Detail-oriented and organized

Education

Master's Degree in Biology

Tools

FIJI ImageJ

Zeiss Zen

Microsoft Office Suite

Job description

Position Objective:

The Biologist will provide support services to satisfy the overall operational objectives of the National Institute on Aging (NIA) within the National Institutes of Health (NIH). The primary objective is to provide research services and deliverables through performance of support services for the Confocal Imaging Facility core of the NIA IRP.

Duties and Responsibilities:

• Study basic principles of plant and animal life, such as origin, relationship, development, anatomy, and function
• Identify, classify, and study structure, behavior, ecology, physiology, nutrition, culture, and distribution of plant and animal species
• Proficiency in Confocal Microscopy image acquisition and analysis 1
• Proficiency in Immunohistochemistry IHC and ICC and immunofluorescence IF techniques 4
• Functional knowledge of super-resolution microscopy techniques 5
• Sample preparation for transmission electron microscopy (TEM); fixation, resin embedding, ultrathin sectioning, post staining, immunogold labeling and negative staining. 2 2
• Preparation of solutions and chemicals 3
• Troubleshooting and optimizing laboratory protocols and instrumentation.
• User training and support.
• Operate laboratory equipment to analyze samples
• Analyze laboratory findings
• Operation of the TEM and support equipment
• Responsible for a clean and organized laboratory.
• User training and support.
• Program and use computers to store, process, and analyze data
• Interpret research or operational data
• Record research or operational data
• Pursue the research goals of the laboratory by conducting wet-lab works
• Work with staff on performing various experiments and procedures
• Harvest and collect mouse embryos, tissues and organs
• Perform histological techniques, including tissue and or whole-mount in situhybridization and immunohistochemistry
• Prepare and organize various lab samples
• Conduct molecular biological analyses
• Provide a broad range of technical assistance to laboratory members
• Maintain laboratory supplies, equipment and experimental records
• Provide support for ordering reagents and equipment required for the laboratory
• Work products and documents related to conducting wet-lab works including harvesting and collecting mouse embryos, tissues and organs; perform histological techniques; conduct various molecular biological analyses.
• Monthly Work products and documents related to maintaining the laboratory’s supplies, equipment and experimental records.
• Clean Equipment, Run Validation, Inspect Equipment, Meet with lab members to present updates, Work products and documents related to training and supervising researchers in confocal microscopy

Qualifications

Basic Qualifications:

• Masters Degree in Biology or a related discipline.
• Skilled in Microsoft office suite, FIJI Image J, Zeiss Zen
• Experienced in Confocal Light Microscopy
• Must have 4 years demonstrated experience in a biological research setting with routine classic embedding and sample preparation techniques including ultramicrotomy for electron microscopy methods. Experience with cryo-fixation preparation techniques for cryo electron microscopy is a plus.
• At least 2-5 years of experience using Scanning Electron Microscopes (SEMs) or Transmission Electron microscopes (TEMs).
• Experience with Focused Ion Beam, Scanning Electron Microscopy or other volumetric electron microscopy techniques is a plus.
• Familiarity with automated imaging systems with either light on electron microscopes in a biological research laboratory.
• Experience with digital imaging of biological systems using light microscopy.
• Familiarity with workflow using 3D reconstruction software

Minimum Qualifications:

• Strong communication skills, both oral and written.
• Detail-oriented and possess strong organizational skills with the ability to prioritize multiple tasks and projects.

This job description is not designed to cover or contain a comprehensive listing of activities, duties or responsibilities that are required by this position.

To perform this job successfully, an individual must be able to perform each essential duty satisfactorily. The requirements listed above are representative of the knowledge, skill, and/or ability required. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

GAP Solutions provides reasonable accommodations to qualified individuals with disabilities. If you need an accommodation to apply for a job us at [e-mail] . You will need to reference the requisition number of the position in which you are interested. Your message will be routed to the appropriate recruiter who will assist you. Please note, this email address is only to be used for those individuals who need an accommodation to apply for a job. Emails for any other reason or those that do not include a requisition number will not be returned.

Qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, protected veteran status or other characteristics protected by law.

This position is contingent upon contract award.