CGT Facility-IMCPL

The University of Pittsburgh; Pittsburgh (Allegheny County); In loco; USD 85.000 - 110.000; Tempo pieno

Descrizione del lavoro

The University of Pittsburgh is seeking a CGT Facility-IMCPL Laboratory Manager IV to direct day-to-day operations in Health Sciences Core Research Facilities on the Pittsburgh campus. This role ensures safety compliance, manages equipment and inventory, and writes grant-related documents.

You will lead cell culture, immunology, analytical development, and flow cytometry programs, guiding collaborations to translate research into scalable, quality-assured processes.

Competenze

• Minimum 7 years of relevant laboratory experience.
• Bachelor's degree required.
• Experience with cell culture, flow cytometry, and immunology methods.
• Ability to develop, validate, and transfer assays.

Mansioni

• Lead development and optimization of primary-cell isolation, enrichment, activation, culture, expansion, harvest, and handling procedures.
• Design studies linking manufacturing conditions to quality attributes and sampling plans.
• Lead development, qualification, validation, and lifecycle management of molecular, cellular, biochemical, and functional methods.
• Lead multiparameter flow cytometry panel design, sample preparation, staining, instrument setup, compensation, gating, and reporting.
• Develop and optimize assays for T-cell activation, proliferation, cytokine release, and cytotoxicity.
• Translate mechanistic assays into reproducible, QC-ready potency methods.

Conoscenze

Cell culture

Flow cytometry

Immunology

Molecular assays

Analytical development

Formazione

Bachelor's Degree

Strumenti

ELISA

ELISpot

Flow cytometry

Descrizione del lavoro

Job Description - CGT Facility-IMCPL (26006513)

CGT Facility-IMCPL

Health Sciences Core Research Facilities - Pennsylvania-Pittsburgh - ( 26006513 )

Job Summary

Directs day-to-day laboratory operations while ensuring compliance with safety regulations and best practices. Works with, adapts, and installs scientific equipment in research laboratories. Develops complex projects and procedures independently. Manages inventory and equipment and performs budget functions. Writes grant applications and manuscripts independently.

Essential Functions

• b. Cell culture and process development. Lead development and optimization of primary-cell isolation, selection, enrichment, activation, culture, expansion, harvest, and handling procedures. Evaluate cell populations derived from blood, bone marrow, tissues, and tumors, including T cells, TILs, dendritic cells, and other relevant immune populations. Assess how culture conditions, media, stimulation strategies, processing steps, and hold conditions affect yield, viability, phenotype, function, and manufacturing consistency. Support transduction, transfection, or electroporation development with appropriately qualified collaborators where required.
• c. Product development and process characterization. Design studies that connect manufacturing conditions and process parameters with critical quality attributes. Establish appropriate sampling plans and in-process measurements, evaluate process variability, and support scale translation and comparability assessments. Use manufacturing and product-characterization data to identify improvements; collaborate with investigators to evaluate relationships with clinical outcomes when suitable data are available.
• d. Analytical development. Lead development, optimization, qualification, validation, execution, troubleshooting, and lifecycle management of molecular, cellular, biochemical, and functional methods. Methods may address safety, identity, purity, impurities, potency, biological activity, gene or transgene expression, residual materials, stability, and vector- or genome-related attributes within assigned program scope. Establish controls, reference materials, system suitability criteria, and standardized data analysis procedures.
• e. Flow cytometry and cell sorting. Lead multiparameter panel design, sample preparation, staining, instrument setup, compensation, gating, assay controls, and reporting. Develop methods for identity, viability, purity, activation, differentiation, and memory phenotypes, including T-cell memory stem populations where relevant. Apply intracellular cytokine staining and CFSE-based proliferation methods. Provide technical leadership for sorting and isolation of defined or rare immune-cell subsets, with appropriate aseptic controls where required.
• f. Functional and immunological assays. Develop and optimize assays for T-cell activation, proliferation, cytokine release, target recognition, and real-time cytotoxicity. Apply ELISA, ELISpot, multiplex assays, ADCC, and other relevant immune-effector methods to product development and characterization. Support assessment of TCR specificity, cross-reactivity, alloreactivity, and tumor-reactive cell function where applicable. Translate relevant mechanistic assays into reproducible, practical potency methods suitable for QC implementation.

Physical Effort

Sitting and standing for long periods. Reading small print on tubes and vials. Walking around campus to attend meetings. Lifting up to 30 pounds. Bending and lifting to put away supplies. Working under standing office and lab lighting.

The University of Pittsburgh is an equal opportunity employer / disability / veteran.

Assignment Category Full-time regular

Job Classification Staff.Laboratory Manager IV

Job Family Research

Job Sub-Family Laboratory

Campus Pittsburgh

Minimum Education Level Required Bachelor's Degree

Minimum Years of Experience Required 7

Will this position accept substitution in lieu of education or experience? Combination of education and relevant experience will be considered in lieu of education and/ or experience requirement.

Work Schedule Monday - Friday, 8:30 a.m. - 5:00 p.m

Work Arrangement On-Campus: Teams that work on campus, in an office, or in a lab.

Hiring Range TBD Based Upon Qualifications

Relocation Offered No

Visa Sponsorship Provided No

Background Check For position finalists, employment with the University will require successful completion of a background check

Child Protection Clearances Not Applicable

The University of Pittsburgh is an equal opportunity employer / disability / veteran.