Biotransformation Scientist

Seventh-Wave-Laboratories-LLC; St. Louis (MO); On-site; USD 110,000 - 170,000; Full time

Benefits offered by this job

Health coverage

Dental coverage

401K participation

Job summary

Inotiv is seeking a Biotransformation Scientist to lead in vitro and ex vivo metabolism studies, identifying enzymatic pathways for small molecules, peptides, and oligonucleotides. The role emphasizes LC-MS/MS and HRMS with a focus on establishing in vitro–in vivo relationships for drug clearance.

You will collaborate with Pharmacology and Toxicology teams to develop NAMs and predictive models for human half-life, supporting ADME programs in a fast-paced research environment.

Qualifications

• PhD in Organic Chemistry, Biochemistry, Analytical Chemistry or related field with 2–5 years of industrial biotransformation experience.
• MSc in a discipline described with 5–7 years direct industrial biotransformation experience.
• Experience with LC-MS/MS and HRMS for quantitation and structure elucidation.
• Knowledge of enzyme kinetics desirable.

Responsibilities

• Design and execute in vitro and ex vivo biotransformation assays using LC–MS/MS/HRMS for quantitation and structure elucidation.
• Elucidate enzymatic pathways and primary clearance mechanisms in vitro and in vivo.
• Collaborate with Toxicology and Pharmacology to establish NAMs and in vitro–in vivo correlations.
• Support ADME studies and PK modeling to predict human half-life and drug interactions.

Skills

LC-MS/MS

HRMS

In vitro metabolism

Biotransformation

Pharmacokinetic modeling

Education

PhD in Organic Chemistry

MSc in Chemistry/Biochemistry

Tools

LC-MS data analysis

Mass spectrometry instrumentation

Wet chemistry separations

Job description

The Biotransformation Scientist will assist with the identification of enzymatic pathways that metabolize drug candidates in preclinical in vitro and in vivo models, including human tissue and cellular models (e.g., P450, non P450/AO/FMO, conjugative/UGT). Working primarily with preclinical species and human tissues/organ subcellular fractions, the individual will utilize contemporary mass spectrometry techniques (LC-MS/MS, HRMS) to identify and elucidate the structures of drug metabolites (small molecules, peptides, oligonucleotides). The individual will contribute to the development of in vitro-to-in vivo relationships related to drug clearance & half-life predictions. The individual will collaborate with Pharmacology and Toxicology laboratory leadership to establish Novel Approach Methodologies (NAMs) to address mechanistic inquiries and/or platform screening processes for same.Join us in embracing research and science to impact the health and well-being of people all over the world.Essential Duties and ResponsibilitiesDesign and execute laboratory studies aimed at identifying the enzymatic pathways that metabolize small molecule, peptide, and oligonucleotide therapeutics employing a contemporary approach methodology to establish the primary enzyme(s) contributing to the in vitro metabolism of a drug candidate(s).Design and execute laboratory studies to elucidate primary drug clearance mechanisms in vitro, employing contemporary approach methodology to establish in vitro to in vivo correlations for anticipated drug clearance towards the prediction of human half life.Design and collaborate with in vivo pharmacology & toxicology team members to execute ADME studies (non radiolabeled); elucidating the disposition of drug candidate molecules as it pertains to absorption, distribution, metabolism, and excretion; supplementing with in vitro protein binding, and blood distributionFor oligonucleotide and peptide molecules, design and execute in vitro studies aimed at elucidating the exonuclease/endonuclease and peptidase(s) metabolism of drug candidates, respectively.Collaborate with the Toxicology Department to establish cellular models of drug metabolism, induction, and predictive toxicologic mechanisms.Skills and AbilitiesExperience conducting in vitro and ex vivo biotransformation assays utilizing LC–MS/MS & HRMS techniques for quantitation and structure elucidation.Experience, skills, and abilities must include knowledge of contemporary bioanalytical techniques, including wet chemistry separations, liquid chromatography, and mass spectrometric analysis, instrument operation, and data processing. Knowledge of enzyme kinetics desirable.Ability to incorporate software in the maintenance of databases to collate and catalog records and inventory.Independence in the laboratory and data work-up phases of research.A working knowledge and awareness of general laboratory procedures.Good written and oral communication skills.Ability to adhere to all safety regulations and procedures.Education and ExperiencePhD in Organic Chemistry, Biochemistry, Analytical Chemistry or related discipline with 2-5 years direct industrial biotransformation experience following Post Doctoral trainingM.Sc. in a discipline previously described with 5-7 years direct industrial biotransformation experienceWorking command of in vitro kinetic analysis and utilization of contemporary mathematical models to effectively analyze, interpret and/or model drug clearance, predicted half life, and drug-drug interactionsAbility to perform mass spectrometry data collection, analysis and interpretation experience via LC-MS/MSCandidates possessing a command of Pharmacokinetic modeling, analysis, interpretation and simulation desirablePhysical Activity and Working ConditionsWork is normally performed in a typical interior office work or laboratory environmentWork environment involves exposure to potentially dangerous materials and situations that require following extensive safety precautions and may include use of protective equipmentThe noise level is usually moderateThis position will frequently be involved with radiant/electrical energy, solvents, grease, oil, irritants, acids, bases, and other hazardous chemicals, electro-mechanical hazards, flammable materials and bio-hazards. Due to exposure to hazardous biological material (which may include HIV positive specimens), immunization to Hepatitis B and/or other diseases may be a requirement.Salary will be commensurate with experience and responsibilities. Benefits include health and dental coverage, short- and long-term disability, paid time off, paid parental leave, 401K participation with company match, and more!Inotiv is an Equal Opportunity Employer. It is our policy to provide a fair and equal employment opportunity to all persons, regardless of age, race, color, religion, gender, gender identity, sexual orientation, physical or mental disability, national origin, veteran status, or any other basis prohibited by law. This policy governs all aspects of employment, including selection, job assignment, compensation, discipline, termination and access to benefits and training.*This position could be offered at different levels for candidates who qualify with a combination of advanced levels of education and/or years of experience. The salary range will adjust along with the level of the role to match the person’s relevant experience and/or education level.Inotiv is a growing contemporary drug discovery and development company where we “Play to Win” in an industry rich with opportunity. Together, we face challenges and together we win in the delivery of world-class drug discovery and development solutions that impact the health and well-being of people all over the world. There’s an opportunity for everyone at all phases of a career, each individual’s unique skill set has an impact on the work we conduct. If you have the talent and desire to impact the quality of people’s lives, we have the career opportunities to make it happen. Join us in embracing research and science to impact the health and well-being of people all over the world.Salary will be commensurate with experience and responsibilities. Benefits include health and dental coverage, short- and long-term disability, paid time off, paid parental leave, 401K, and more!Inotiv is an Equal Opportunity Employer. It is our policy to provide a fair and equal employment opportunity to all persons, regardless of age, race, color, religion, sex, gender, gender identity, gender expression, sexual orientation, genetic information, disability, national origin, veteran status, or any other basis prohibited by law. This policy governs all aspects of employment, including selection, job assignment, compensation, discipline, termination and access to benefits and training.