Associate Scientist, AAV Vector Production & QC

Manifold Bio; Boston (MA); On-site; USD 100,000 - 115,000; Full time

Benefits offered by this job

Stock options

Medical, dental, and vision coverage

401(k) plan

Paid time off and holidays

On-site gym

Onsite lunch

Commuter support

Annual performance-based bonus

Job summary

Manifold Bio is seeking an Associate Scientist with hands-on expertise in adeno-associated virus (AAV) vector production, purification, and analytical characterization to join our team. You will generate high-quality, well-characterized AAV lots for rodent and NHP in vivo studies and scale transient transfection from small to large volumes.

You will optimize downstream purification (affinity/ion-exchange chromatography, TFF) and perform key assays (qPCR/ddPCR, ELISA, HPLC, SDS-PAGE, CGE,

Qualifications

• B.S. or M.S. in a biological field with 2–5 years of industry experience.
• Hands-on AAV production in HEK293, triple transfection, from ≤1 L to ≥50 L scale.
• Proficiency with downstream purification techniques incl. affinity & ion‑exchange chromatography.

Responsibilities

• Produce, purify, and qualify AAV vector lots for study-ready material.
• Maintain and troubleshoot cell culture systems.
• Develop and execute QC assays; analyze, interpret, and report data.
• Drive process-optimization experiments to improve yield and quality.
• Process ex‑vivo tissues for NGS analysis.
• Communicate progress and results to cross-functional teammates.

Skills

AAV vector production

Purification & QC assays

Molecular biology techniques

Education

B.S. or M.S. in Biochemistry, Molecular Biology, Chemical Engineering, Virology, or related field

Tools

AKTA-FPLC

Affinity chromatography

Ion-exchange chromatography

HPLC

NGS library prep

Job description

Manifold Bio is seeking an Associate Scientist with hands-on expertise in adeno-associated virus (AAV) vector production, purification, and analytical characterization to join our team. You will generate high-quality, well-characterized AAV lots for rodent and NHP in vivo studies and scale transient transfection from small to large volumes.

You will optimize downstream purification (affinity/ion-exchange chromatography, TFF) and perform key assays (qPCR/ddPCR, ELISA, HPLC, SDS-PAGE, CGE,