Associate Scientist

Flagship Pioneering, Inc.; Cambridge (MA); On-site; USD 96,000 - 127,000; Full time

Job summary

Quotient Therapeutics is seeking an Associate Scientist with experience in molecular biology, immunology, and early drug discovery to advance translational validation in inflammatory and autoimmune disease models.

The role involves designing in vitro assays, executing cell-based and biochemical experiments, and interpreting data to guide go/no-go decisions within a dynamic, cross-functional team.

Qualifications

• BS or MS in Immunology, Biochemistry, Molecular Biology, or a related discipline with 3+ years of industry experience for MS or 5+ years for BS.
• In-depth understanding of cellular and molecular biology techniques including mammalian cell culture, transfection, CRISPR, western blots, qPCR, immunofluorescence and flow cytometry.
• Prior experience running cell-based compound screening assays.
• Prior experience in an industrial setting, and familiarity with GLP as well as proficiency in ELN.
• Experience with experimental design and strong proficiency in assay execution, troubleshooting, data analysis, and data interpretation.
• Outstanding organizational and record keeping skills.
• Execution focused, team oriented, and highly motivated.

Responsibilities

• Design, optimize and implement robust in vitro experiments to validate target mechanisms and translational relevance to human disease.
• Execute biochemical and cell-based assays using recombinant proteins, mammalian cell lines and primary cells to define drug mechanism of action.
• Innovate, troubleshoot and interpret data to drive go/no go decisions.
• Maintain precise experimental records and documentation.
• Contribute to preparation of research articles, reports and presentations.
• Follow lab safety procedures and participate in regular team meetings.

Skills

Mammalian cell culture

Transfection

CRISPR

Western blot

qPCR

Immunofluorescence

Flow cytometry

Cell-based screening assays

Data analysis

Research design

Education

BS/MS in Immunology, Biochemistry or Molecular Biology

Tools

ELN

Job description

Position Summary:

Quotient is seeking an Associate Scientist with experience in molecular biology, immunology and early drug discovery. This position will contribute to the translational validation of discovery platform targets in mechanistic and disease-relevant pharmacological models.

The ideal candidate will be a talented, goal-oriented and highly motivated individual who will play a vital role in progressing the company's early discovery projects in inflammatory and autoimmune diseases.

The candidate must be comfortable working in and contributing to a diverse, highly dynamic, fast-paced and cross-functional team environment.

Responsibilities:

• Use insights from literature and experimental results to design, optimize and implement robust in vitro experiments to validate target mechanisms and translational relevance to human disease
• Execute biochemical and cell-based assays using recombinant proteins, mammalian cell lines and primary cells to define drug mechanism of action
• Utilize creativity and critical thinking skills to innovate, troubleshoot/optimize experiments and to interpret data to drive go/no go decisions
• Must display attention to detail, sense of urgency and "outside the box" thinking
• Work under the supervision of senior members of the team and assist in designing and implementing research protocols
• Perform laboratory experiments and apply various laboratory techniques including compound screens, flow cytometry, ELISA, qPCR, Western blotting.
• Conduct in vitro and ex vivo assays, such as but not limited to overexpression, knockdown, knockout on immortalized and primary cells, stimulation, and cytokine profile or functional assays
• Collect, analyze and interpret scientific data accurately
• Maintain precise experimental records and documentation
• Contribute to the preparation of research articles, reports and presentations
• Follow lab safety procedures and rules to ensure a safe working environment
• Participate in regular team meetings to discuss progress, challenges, and future plans

Qualifications:

Essential

• BS or MS in Immunology, Biochemistry, Molecular Biology, or a related discipline and 3+ years of industry experience for MS or 5+ years for BS
• In depth understanding of cellular and molecular biology techniques including mammalian cell culture, transfection, CRISPR, western blots, qPCR, immunofluorescence and flow cytometry
• Prior experience running cell-based compound screening assays
• Prior experience in an industrial setting, and familiarity with Good Laboratory Practice (GLP) as well as proficiency in the use of Electronic Laboratory Notebooks (ELN)
• Experience with experimental design and strong proficiency in assay execution, troubleshooting, data analysis, and data interpretation
• Outstanding organizational and record keeping skills
• Execution focused, team oriented, and highly motivated

Desirable

• Proficiency with RNA-seq, cloning and image analysis
• Previous experience with lentiviral transductions or AAV transfection/transduction

Values and Behaviors:

• Encourage respectful disagreement and cultivate open-minded, ego-free interactions to continuously push each other towards excellence
• Seek out diverse perspectives; practice active listening and genuine curiosity to ensure all contributions are valued, regardless of source
• Recognize the impact of your behavior, language and attitudes, and strive for balanced, meaningful exchanges that enhance mutual growth and understanding in all interactions
• Use a company-first mindset to guide decision-making; prioritize team over individual success
• Take calculated risks and challenge convention in the quest for exceptional outcomes

About Quotient:

Quotient Therapeutics is a privately-held, early stage company developing breakthrough medicines informed by natural somatic genetic diversity present in patients. Through our work in somatic genomics, we are forging a new status quo for biopharma research and development across a broad pipeline of internal and partnered programs.

The company was founded by Flagship Pioneering, an innovative enterprise that conceives, creates, resources, and grows first-in-category life sciences companies. Flagship Pioneering has created over 100 groundbreaking companies over the past twenty years, all of which are pioneering novel and proprietary biological, industrial, and engineering approaches to solve major needs in human health and sustainability. These companies include Moderna (MRNA), Generate Biomedicines, Sana Biotechnology (SANA), Tessera Therapeutics, Evelo Biosciences (EVLO), Indigo Agriculture, Seres Therapeutics (MCRB), and Syros Pharmaceuticals (SYRS).

The salary range for this role is $96,000 - $126,500. Compensation for the role will depend on a number of factors, including a candidate’s qualifications, skills, competencies, and experience. Quotient Therapeutics, Inc. currently offers healthcare coverage, annual incentive program, retirement benefits and a broad range of other benefits. Compensation and benefits information is based on Quotient Therapeutics, Inc.'s good faith estimate as of the date of publication and may be modified in the future.