Antibody Discovery & Engineering Lab Specialist

The University of Pittsburgh; Pittsburgh (Allegheny County); In loco; USD 55.000 - 75.000; Tempo pieno

Descrizione del lavoro

The University of Pittsburgh's Center for Antibody Therapeutics (CAT) within the Division of Infectious Diseases seeks a Laboratory Research Specialist to advance antibody discovery, engineering, and development programs. Responsibilities include cloning, expression, purification, and flow-cytometry based characterization in collaboration with faculty and trainees.

The role emphasizes data integrity, regulatory compliance, and strong teamwork to support externally funded projects and routine lab

Competenze

• Bachelor's Degree required.
• Minimum 2 years of related laboratory experience.
• Experience in antibody discovery or engineering is preferred.
• Ability to work collaboratively with faculty, staff and students.

Mansioni

• Perform molecular cloning and recombinant DNA techniques for antibody constructs.
• Support antibody engineering via design, humanization, and maturation.
• Express antibodies in bacterial and mammalian systems and maintain cell cultures.
• Purify/characterize antibodies using Protein A/G chromatography, SDS-PAGE and ELISA.
• Conduct flow cytometry assays for binding, specificity, potency and receptor occupancy.
• Document data, prepare reports, figures, and summaries for investigators.
• Maintain inventories, cell banks, reagents, and safety-compliant records.
• Assist with equipment maintenance, troubleshooting, and laboratory operations.
• Collaborate with faculty, researchers, and students on externally funded programs.

Conoscenze

Team collaboration

Attention to detail

Regulatory compliance

Documentation

Data analysis

Experiment design

Formazione

Bachelor's Degree

Strumenti

Molecular cloning

Recombinant antibody expression

Flow cytometry

Protein A/G chromatography

SDS-PAGE

ELISA

Transient transfection

Cell culture

Descrizione del lavoro

The University of Pittsburgh's Center for Antibody Therapeutics (CAT) within the Division of Infectious Diseases seeks a Laboratory Research Specialist to advance antibody discovery, engineering, and development programs. Responsibilities include cloning, expression, purification, and flow-cytometry based characterization in collaboration with faculty and trainees.

The role emphasizes data integrity, regulatory compliance, and strong teamwork to support externally funded projects and routine lab