Analytical Scientist II

Randstad USA; St. Louis (MO); On-site; USD 37,000 - 50,000; Full time

Job summary

MilliporeSigma’s Life Sciences R&D facility in St. Louis is seeking a Scientist II to join the Analytical R&D Department.

You will lead the development of LC-MS assays for quantifying proteins in complex mixtures and perform advanced characterization of large biomolecules including monoclonal antibodies, multispecifics, and antibody-drug conjugates. This is a 6-month contract with potential extension, schedule is Monday–Friday 8 AM–5 PM, and compensation ranges from $27 to $36 per hour.

Qualifications

• BS in Chemistry or related field.
• Experience in analytical lab with reagent and sample preparation and instrumental analysis.
• Ability to work independently and in a team-oriented environment.
• Proficiency with LC-MS/HRMS and HPLC instrumentation; strong data reporting skills.
• Hands-on experience with enzymatic and chemical sample prep for large biomolecule LC-MS.

Responsibilities

• Lead development of LC-MS assays for quantitation of proteins in complex mixtures.
• Characterize large biomolecules including monoclonal antibodies, multispecifics, and ADCs.
• Provide high-level analytical support to product development, manufacturing and project teams.

Skills

Analytical laboratory experience

Team communication

LC-MS sample analysis

HPLC proficiency

Education

BS in Chemistry or related field

PhD in Analytical Chemistry or related field

Tools

Empower

Chromeleon

UNIFI

BioPharma Finder

Mascot

Scaffold

Thermo mass spectrometers

Sciex mass spectrometers

Waters mass spectrometers

Agilent mass spectrometers

MALDI-MS

Job description

***THIS IS A 6-MONTH CONTRACT WITH POSSIBILITY OF EXTENSION***

Schedule: M-F 8AM-5PM

Pay: $27-36/hr

Overview

A career with MilliporeSigma is an ongoing journey of discovery: our 56,000 people are shaping how the world lives, works and plays through next generation advancements in healthcare, life science and performance materials. For more than 350 years and across the world we have passionately pursued our curiosity to find novel and vibrant ways of enhancing the lives of others. MilliporeSigma is a business of Merck KGaA, Darmstadt, Germany.

Your Role:

The Scientist II will join the Analytical R&D Department at MilliporeSigma at our Life Sciences R&D facility in St. Louis. In this role, you will be part of an active, multidisciplinary group of scientists dedicated to providing high level analytical support to product development scientists in R&D, manufacturing groups, and special project teams. You will be expected to lead development of LC-MS assays for the quantitation of proteins in complex mixtures. Additionally, you will perform advanced characterization of large biomolecules including monoclonal antibodies, multispecifics, and antibody drug conjugates.

Minimum Qualifications:

• BS in Chemistry or related field
• Experience working in analytical laboratory, performing reagent and sample preparation as well as instrumental analysis and reporting
• The ability to work and communicate effectively both independently and in a team-oriented environment

Preferred Qualifications:

• Demonstrated hands-on proficiency with HPLC instrumentation and chromatographic software such as Empower and Chromeleon
• Strong experience with a variety of enzymatic and chemical methods of sample preparation for large biomolecule LC-MS, such as digestion, enrichment, depletion, and labelling

Ideal Qualifications:

• PhD in Analytical Chemistry or related field
• Expertise in absolute quantitation of proteins and antibodies by Multiple Reaction Monitoring (MRM) and/or Parallel Reaction Monitoring (PRM) mass spectrometry
• Expertise in bottom up, middle up/down, and top down protein LC-MS workflows
• Experience with specific workflows such as non-reduced and reduced intact mass analysis, peptide mapping, disulfide mapping, Post-Translational Modifications (PTM) characterization, glycosylation profiling, and free thiol analysis
• Expertise with bioinformatics related to the quantitation and characterization of large biomolecules such as UNIFI, BioPharma Finder, Mascot, and Scaffold
• Ideal candidate would have proficiency in a variety of instrument platforms such as Thermo, Sciex, Waters, and Agilent mass spectrometers
• In addition to ESI-MS techniques the ideal candidate would also have hands-on experience with MALDI-MS