AAV Genome Size Quantitation Scientist

National Institute of Standards and Technology; Gaithersburg, Northern (MD, KY); Hybrid; USD 70,000 - 100,000; Full time

Job summary

National Institute of Standards and Technology (NIST) is seeking a PREP associate to advance a project on novel measurement methods for AAV gene therapy vectors. You will help characterize partially filled capsids using advanced microscopy and analytics, and contribute to quality control methods for the gene therapy industry.

The role emphasizes producing, analyzing, and documenting results, with opportunities to present at internal meetings and scientific conferences.

Qualifications

• Ph.D. in Biological Sciences, Biochemistry, Biophysics, or closely related field.
• Proficiency in qPCR, ddPCR, ELISA, and protein/nucleic acid quantification.
• Experience in quantitative data analysis and correlating results from multiple analytical methods.
• Experience in Transmission Electron Microscopy and Atomic Force Microscopy.
• Experience in BSL-2 sample handling.
• Strong oral and written communication skills with publications/presentations.
• Ability to work independently and collaboratively within a multi-disciplinary team.

Responsibilities

• Participating in the production and purification of Adeno-Associated Virus (AAV) samples.
• Performing characterization of AAV preparations using ddPCR, ELISA, and SEC.
• Performing cryogenic electron microscopy (CryoEM) analysis on AAV samples.
• Conducting atomic force microscopy (AFM) and chemical force microscopy (CFM) experiments.
• Analyzing and correlating data from CryoEM, AUC, and AFM to relate genome size, filling state, and capsid hydrophobicity.
• Adhering to biosafety protocols for AAVs and human cell lines.
• Presenting results at internal meetings and potentially at scientific conferences.
• Publication of results in peer-reviewed journals.
• Ensuring that results, protocols, and data analysis are thoroughly documented and archived.

Skills

qPCR

ddPCR

ELISA

Protein quantification

Nucleic acid quantification

Data analysis

TEM

AFM

BSL-2 handling

Communication

Teamwork

Education

Ph.D. in Biological Sciences or related field

Tools

CryoEM

AFM

Job description

National Institute of Standards and Technology (NIST) is seeking a PREP associate to advance a project on novel measurement methods for AAV gene therapy vectors. You will help characterize partially filled capsids using advanced microscopy and analytics, and contribute to quality control methods for the gene therapy industry.

The role emphasizes producing, analyzing, and documenting results, with opportunities to present at internal meetings and scientific conferences.