Research Assistant in Epigenetics of smoking in Multiple Sclerosis

Karolinska Institutet; Stockholms kommun; Vor Ort; SEK 150.000 - 210.000; Teilzeit

Zusammenfassung

Karolinska Institutet in Solna seeks a part-time Research Assistant to investigate epigenetics of smoking in Multiple Sclerosis. You will join a collaborative team of PhD students and senior researchers, performing NGS library preparation and CRISPR-based editing, with bioinformatics support to interpret transcriptional changes.

The role emphasizes translational research, independence, and collaboration in an international environment at a leading medical university.

Qualifikationen

• Master’s degree in a relevant field.
• Background in human diseases, autoimmunity and nervous processes.
• Knowledge of gene regulation and epigenetics.
• Experience with basic molecular and cellular work (cloning, DNA/RNA extraction, qPCR, cell culture, transfection).
• Practical experience with NGS library preparation, including DNA methylation and gene expression.
• Programming in R for NGS-derived analyses in bulk and single-cell RNA sequencing.
• Good command of spoken and written English.

Aufgaben

• Decipher MS pathogenesis with a focus on smoking impact on neuroinflammation.
• Perform NGS library prep on clinical and experimental samples.
• Conduct CRISPR/dCas9-epigenome editing in vitro and analyse results.
• Carry out bioinformatic analyses under guidance of senior bioinformatician.

Kenntnisse

NGS library prep

DNA methylation

RNA sequencing

CRISPR/dCas9 epigenome editing

R programming

Cell culture

qPCR

DNA/RNA extraction

Transfection

English communication

Ausbildung

Master’s degree in a relevant field

Jobbeschreibung

Research Assistant in Epigenetics of smoking in Multiple Sclerosis

Do you want to contribute to top quality medical research?

Join the dynamic and cooperative group of Prof. Maja Jagodic under the supervision of the Team Leader Dr. Lara Kular at the Department of Clinical Neuroscience. This offers the opportunity to contribute to interactive research driven by pertinent clinical questions, combining deep molecular understanding of the mechanisms underlying Multiple Sclerosis (MS) with some of the most competitive clinical materials worldwide. You will benefit from a highly collaborative, engaged and international research environment committed to exploring innovative translational ideas in precision medicine where you will have the possibility to conduct research at the top international level in the field.

Your mission

We are looking for a 50% research assistant for a 6-month position with possibility of extension. The successful candidate will join our research team and collaborate with PhD students, postdocs, and senior researchers to decipher multiple sclerosis pathogenesis, with a focus on the mechanisms underlying the impact of smoking on neuroinflammation in MS. The work entails both an experimental side including next-generation sequencing (NGS) library preparation of clinical and experimental samples, functional experiments based on CRISPR/dCas9-epigenome editing in vitro and bioinformatic work to address transcriptomic changes under the guidance of our senior bioinformatician.

Your profile

We are looking for a candidate who shares our passion for translational research and epigenetics. The candidate should have the ability to work independently as well as collaboratively with colleagues and students.

Absolute requirements:

• Master’s degree in a relevant field.
• Scientific and/or research background on human diseases and mechanisms involved in autoimmunity and nervous processes.
• Knowledge of mechanisms of gene regulation and epigenetics.
• Technical skills in basic molecular (cloning, DNA/RNA extraction, qPCR) and cellular (cell culture, transfection) work.
• Documented practical experience with NGS-based library preparation, preferably in the field of both DNA methylation and gene expression.
• Documented programming skills (preferably in R) in NGS-derived analyses preferably in both bulk and single-cell RNA sequencing.
• Good command of spoken and written English.

Meritorious requirements:

• Knowledge of multiple sclerosis disease
• Practical and theoretical knowledge of the molecular impact of exposure to pollutants such as cigarette smoke (e.g., human omics, in vitro exposure)
• Practical experience with CRISPR-based methylation editing
• Co-authorship of scientific publications or manuscripts is a plus

What do we offer?

A creative and inspiring environment with wide-ranging expertise and interests. Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is also a state university, which entitles you to several good benefits through our collective agreement. And you get to practice freely in our modern wellness facilities, where trained staff are on site.

Location: Solna

Want to make a difference? Join us and contribute to better health for all!