PhD Candidate — Epigenetic T Cell Immunotherapy Research

SIGRA; Amsterdam; On-site; EUR 43,000 - 52,000; Full time

Benefits offered by this job

Travel allowance

Free parking at NKI

AVL Academy education

Job summary

Netherlands Cancer Institute is seeking a PhD candidate to join our team studying mechanisms and functions of epigenetic pathways in T cells, with a focus on how DOT1L reprograms cytotoxic CD8 T cells to potentially enhance anti-tumor responses. The project uses CRISPR-Cas9 genome engineering, epigenetic editing, epigenomics and transcriptomics, and DNA-barcode based screens.

You will validate findings in primary human CD8 T cells and collaborate across divisions, with a four-year contract and a

Qualifications

• MSc in molecular biology or related field.
• Experience with basic molecular biology techniques.
• Affinity for computational biology.
• Fluent in academic English.
• Prior experience in gene regulation or chromatin biology is a plus.
• Interest in epigenetic mechanisms and cancer immunology.

Responsibilities

• Apply CRISPR-Cas9 genome engineering and epigenetic editing in human T cell models.
• Validate mechanisms in primary human CD8 T cells.
• Gain insights to guide epigenetic perturbation to enhance cytotoxic activity towards cancer cells.

Skills

Fluent English

Team collaboration

Problem solving

Academic writing

Proactive mindset

Education

MSc in molecular biology or related discipline

Tools

CRISPR-Cas9

Epigenomics techniques

DNA barcoding screens

Job description

Netherlands Cancer Institute is seeking a PhD candidate to join our team studying mechanisms and functions of epigenetic pathways in T cells, with a focus on how DOT1L reprograms cytotoxic CD8 T cells to potentially enhance anti-tumor responses. The project uses CRISPR-Cas9 genome engineering, epigenetic editing, epigenomics and transcriptomics, and DNA-barcode based screens.

You will validate findings in primary human CD8 T cells and collaborate across divisions, with a four-year contract and a