Scientist - Antibody Conjugation

Yashraj Biotechnology Ltd.; Navi Mumbai; On-site; INR 700,000 - 1,100,000; Full time

Job summary

Yashraj Biotech in Navi Mumbai is seeking a highly motivated Scientist with 4-5 years of industrial experience in antibody bioconjugation, immunoassay development, and diagnostic reagent development. The role focuses on developing and optimizing antibody-latex conjugates for immunoturbidimetric assays while collaborating with RD and Quality teams to deliver robust diagnostic products.

The candidate will design and optimize purification, conjugation workflows, and analytical validation,

Qualifications

• 4–5 years of industrial experience in antibody bioconjugation, immunoassays, and diagnostic reagents.
• Design, optimize purification, conjugation, and immunoassay workflows.
• GLP-compliant documentation and development records.
• Mentor junior scientists and collaborate with RD and QA teams.

Responsibilities

• Design, execute, and optimize antibody purification and bioconjugation workflows.
• Develop EDC/NHS covalent conjugation strategies for latex and nanoparticle-based diagnostic reagents.
• Optimize antibody loading, blocking, coupling chemistry, and conjugate stability.
• Develop and optimize immunoturbidimetric assays (ITA) and DOE-based optimization studies.
• Evaluate analytical performance including sensitivity, specificity, linearity, precision, dynamic range, and robustness.
• Troubleshoot conjugation, assay performance, and reagent stability issues.
• Maintain accurate experimental documentation and SOPs in GLP compliance.
• Mentor junior scientists, trainees, and interns in laboratory techniques.
• Participate in technology transfer activities and support manufacturing scale-up.

Skills

Antibody bioconjugation

Immunoassay development

Diagnostic reagent development

DOE optimization

GLP documentation

Mentoring

Cross-functional collaboration

Antibody purification

Nanoparticle conjugation

Conjugation chemistry

Tools

EDC/NHS chemistry

Maleimide-thiol chemistry

Glutaraldehyde coupling

Latex bead conjugation

Nanoparticle conjugation

Dialysis and ultrafiltration

DLS

Zeta potential analysis

UV-Vis spectrophotometry

Job description

Yashraj Biotech is seeking a highly motivated Scientist with 4-5 years of industrial experience in antibody bioconjugation, immunoassay development, and diagnostic reagent development. The candidate will work in the development and optimization of antibody-latex conjugates for immunoturbidimetric assays while collaborating with multidisciplinary RD and Quality teams to deliver robust diagnostic products.

Key Responsibilities

• Design, execute, and optimize antibody purification, characterization, and bioconjugation workflows.
• Develop EDC/NHS-based covalent conjugation strategies for latex and nanoparticle-based diagnostic reagents.
• Optimize antibody loading, blocking conditions, coupling chemistry, and conjugate stability.
• Develop and optimize immunoturbidimetric assays (ITA)
• Design and execute DOE-based optimization studies for conjugation efficiency and assay performance.
• Evaluate analytical performance including sensitivity, specificity, linearity, precision, dynamic range, and robustness.
• Troubleshoot conjugation, assay performance, and reagent stability issues.
• Maintain accurate experimental documentation, SOPs, technical reports, and development records in compliance with GLP.
• Mentor junior scientists, trainees, and interns in laboratory techniques and experimental planning.
• Participate in technology transfer activities and support manufacturing scale-up of developed reagents.

Cross-Functional Responsibilities

• Collaborate with Polyclonal and Monoclonal Antibody RD teams for antibody selection and characterization.
• Work closely with Quality Control (QC) during analytical validation and product performance evaluation.
• Coordinate with Quality Assurance (QA) to ensure compliance with documentation, change control, and regulatory requirements.
• Support Manufacturing in process optimization and troubleshooting during scale-up.
• Participate in product development meetings, technical reviews, and root-cause investigations.
• Contribute to continuous process improvement and new technology implementation.

Required Technical Skills

Antibody Protein Sciences

• Strong understanding of monoclonal, polyclonal, artificial polyclonal antibodies.
• Knowledge of antibody structure, affinity, avidity, stability, and antigen-antibody interaction kinetics.
• Experience handling native and recombinant antigens and antibodies.
• Hands-on experience in antibody purification using ammonium sulphate precipitation, dialysis, ultrafiltration, and related techniques.
• Understanding of protein stability and conjugate characterization.

Bioconjugation

• Hands-on industrial experience in EDC/NHS chemistry, Maleimide-Thiol Chemistry, Glutaraldehyde Coupling
• Expertise in antibody immobilization onto latex beads , nanoparticles
• Understanding of surface chemistry, functional group activation, antibody orientation, and conjugation efficiency
• Experience in optimization of conjugation parameters for improved assay performance

Latex/Nanoparticle Diagnostics

• Experience developing latex-based diagnostic reagents.
• Understanding of particle size selection, surface modification, colloidal stability, blocking strategies, and storage stability.
• Knowledge of reagent formulation and long-term stability assessment.

Immunoassays

• Hands‑on experience with Immunoturbidimetric Assays (ITA)
• Working knowledge of ELISA and CLIA platforms
• Experience in assay optimization, calibration curve generation, cut-off determination, and analytical validation

Analytical Techniques

Experience with:

• Immunoturbidometry
• UV-Visible Spectrophotometry
• Downstream processing of polyclonal antibodies
• Dynamic Light Scattering (DLS)
• Zeta potential analyser
• Dialysis and ultrafiltration
• Statistical analysis of assay performance

Laboratory Practices

• Excellent laboratory skills including precision pipetting and sample handling.
• Experience working under GLP/GMP environments.
• Knowledge of laboratory safety, contamination control, and documentation practices.

Desired Competencies

• Strong experimental design and scientific problem-solving skills.
• Excellent troubleshooting and analytical thinking.
• Ability to independently manage multiple RD projects.
• Strong written and verbal scientific communication.
• Experience mentoring junior team members.
• Good organizational and project management skills.
• Ability to work effectively in cross‑functional teams.

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