Stem Cell Research Assistant: hPSC Culture & Analysis

The University of Sheffield; Sheffield; On-site; GBP 32,000 - 33,000; Full time

Benefits offered by this job

Annual leave

Pension scheme

Flexible working

Wellbeing support

Discounts

Job summary

The University of Sheffield is seeking a Research Assistant to support routine human pluripotent stem cell culture, differentiation and analysis within the Tsakiridis Lab. You will manage sample prep, data analysis and general lab duties to support a multi-year MRC DPFS project on cell therapy development.

Applicants should hold a BSc/MSc in Stem Cell/Developmental Biology with hands-on experience in qPCR, immunostaining, flow cytometry and fluorescence microscopy, plus strong IT and

Qualifications

• BSc or MSc in a relevant biological discipline such as Stem Cell/Developmental Biology.
• Experience with cell (ideally hPSC) culture, RNA isolation and qPCR.
• Immunostaining, fluorescence/confocal microscopy and flow cytometry experience.
• Strong IT skills and knowledge of laboratory software; good organisation and planning.
• Ability to prioritise and plan workload to deadlines, with attention to detail.
• Experience in qPCR, immunostaining, flow cytometry and fluorescence/confocal microscopy.

Responsibilities

• Routinely culture, differentiate and harvest hPSC lines on weekdays and weekends.
• Analyse samples by immunocytochemistry, flow cytometry and qPCR.
• Assist in data analysis and interpretation; record results accurately.
• Support lab management tasks: stock, reagents, equipment upkeep.
• Contribute to manuscript writing and dissemination of research findings.
• Coordinate with PI and staff to plan future work and timelines.
• Help ensure safety training and regulatory compliance in the lab.

Skills

Stem cell biology

hPSC culture

qPCR

Immunostaining

Flow cytometry

Lab IT skills

Data analysis

Education

BSc or MSc in Stem Cell/Developmental Biology

Tools

Laboratory software

Job description

The University of Sheffield is seeking a Research Assistant to support routine human pluripotent stem cell culture, differentiation and analysis within the Tsakiridis Lab. You will manage sample prep, data analysis and general lab duties to support a multi-year MRC DPFS project on cell therapy development.

Applicants should hold a BSc/MSc in Stem Cell/Developmental Biology with hands-on experience in qPCR, immunostaining, flow cytometry and fluorescence microscopy, plus strong IT and