PhD Researcher: Mapping lncRNA Translation in Neurons

ORFeus doctoral network; Leeds; On-site; GBP 55,000 - 59,000; Full time

Job summary

ORFeus doctoral network invites applications for a full-time MSCA-funded doctoral position in the United Kingdom. The project investigates how lncRNAs are translated, combining TCP-Seq and long-read direct-RNA-Seq in SH-SY5Y cells, supported by a networked team across ORFeus and partner projects.

You will join a supervisory team at the University of Leeds and undertake a three-month secondment with Oxford Nanopore Technologies, with a PhD enrollment leading to a doctoral degree.

Qualifications

• Hands-on laboratory experience in molecular and RNA biology, e.g. mammalian cell culture, cloning and reporter assays, or RNA sequencing methods.
• Desirable: experience with translation assays, next-generation or long-read sequencing, basic bioinformatics or scripting (R or Python).
• Interest in microproteins and the dark proteome; good written and spoken English; IELTS/TOEFL acceptable.

Responsibilities

• Perform TCP-Seq and map ribosome scanning during neuronal differentiation.
• Identify transcript features controlling ribosome recruitment using long-read direct-RNA-Seq and mutation analyses.
• Validate regulatory factors and motifs; compile a genome-wide map of ribosome scanning across lncRNAs.
• Collaborate across the ORFeus network and contribute to the project report and models.

Skills

Molecular biology techniques

RNA sequencing methods

Bioinformatics basics

Scripting (R/Python)

Translation assays

Education

Molecular biology/biochemistry/RNA biology background

Job description

ORFeus doctoral network invites applications for a full-time MSCA-funded doctoral position in the United Kingdom. The project investigates how lncRNAs are translated, combining TCP-Seq and long-read direct-RNA-Seq in SH-SY5Y cells, supported by a networked team across ORFeus and partner projects.

You will join a supervisory team at the University of Leeds and undertake a three-month secondment with Oxford Nanopore Technologies, with a PhD enrollment leading to a doctoral degree.