iPSC Neuroscience Technician – On-Site

Dunhillmedical; Sheffield; Hybrid; GBP 32,000 - 37,000; Full time

Benefits offered by this job

Highly competitive annual leave

Generous pensions scheme

Flexible working opportunities

Benefits discounts

Job summary

The University of Sheffield’s SITraN invites applications for a Research Technician to support Friedreich’s ataxia research. You will maintain and differentiate human iPSC cultures and contribute to cellular and molecular studies in a multidisciplinary neuroscience setting.

Working with a postdoctoral researcher, you will perform ICC, fluorescence microscopy and RNA techniques, keeping rigorous records and supporting data analysis for publications.

Qualifications

• Minimum MSc in Neuroscience or related subject; or Honours degree in Neuroscience or related subject.
• Demonstrable experience with induced pluripotent stem cell (iPSC) models.
• Experience working in a collaborative research team.
• Experience maintaining iPSC in culture and deriving iPSC neurons.
• Experience in ICC, fluorescence microscopy, RNA extraction and molecular biology techniques.
• Experience in patch-clamp electrophysiology.

Responsibilities

• Provide day-to-day technical support to the postdoctoral researcher and laboratory team.
• Maintain and differentiate iPSC cultures, generating neuronal cell types with consistency.
• Perform immunocytochemistry, fluorescence microscopy, RNA extraction and related techniques.
• Support data collection, analysis and preparation of reports or publications.
• Help develop, optimise and validate laboratory protocols.
• Maintain equipment, reagents and cell culture facilities.

Skills

iPSC work

Team collaboration

Record keeping

Education

MSc in Neuroscience or related subject

Honours degree in Neuroscience or related subject

Tools

Immunocytochemistry

Fluorescence microscopy

RNA extraction

Molecular biology techniques

Patch-clamp electrophysiology

Job description

The University of Sheffield’s SITraN invites applications for a Research Technician to support Friedreich’s ataxia research. You will maintain and differentiate human iPSC cultures and contribute to cellular and molecular studies in a multidisciplinary neuroscience setting.

Working with a postdoctoral researcher, you will perform ICC, fluorescence microscopy and RNA techniques, keeping rigorous records and supporting data analysis for publications.