Cryo-EM Structural Biologist — Research Technician

UKRI International; Greater London; Hybrid; GBP 37,000 - 39,000; Full time

Job summary

The Research Technician – Cryo-EM / Structural Biology at MRC Laboratory of Medical Sciences (LMS) and Imperial College London is a 12‑month fixed‑term, full‑time post based in Hammersmith, London. You will lead the structural arm of a translational cryo‑EM program, producing STAG1 and STAG1‑containing cohesin complexes and determining structures with screening hits.

The ideal candidate will have hands-on single‑particle cryo‑EM experience, ideally with SMC protein complexes, and a PhD in

Qualifications

• PhD in structural biology, biochemistry or a closely related field with a focus on cryo-EM (or a Masters with equivalent research experience)
• Hands-on experience of single-particle cryo-EM, from sample and grid preparation through data collection and processing (e.g. RELION)
• Experience working with SMC protein complexes (cohesin, condensin or SMC5/6)
• Expression and purification of recombinant proteins, ideally large multi-subunit complexes

Responsibilities

• Express and purify recombinant STAG1, cohesin and related SMC complexes for structural and biophysical studies
• Prepare, screen and optimise cryo-EM grids and collect cryo-EM data
• Carry out single-particle image processing, 3D reconstruction, model building and refinement
• Determine structures of STAG1/cohesin complexes with screening hits and define binding modes
• Contribute to hit validation using biochemical and biophysical assays of compound binding
• Collaborate with medicinal chemists and external partners to inform compound design
• Plan and carry out research with appropriate supervision
• Maintain accurate records of work and deposit structural data

Skills

cryo-EM

SMC complexes

Protein expression

Single-particle analysis

Education

PhD in structural biology

Tools

RELION

Cryo-EM data collection

Job description

The Research Technician – Cryo-EM / Structural Biology at MRC Laboratory of Medical Sciences (LMS) and Imperial College London is a 12‑month fixed‑term, full‑time post based in Hammersmith, London. You will lead the structural arm of a translational cryo‑EM program, producing STAG1 and STAG1‑containing cohesin complexes and determining structures with screening hits.

The ideal candidate will have hands-on single‑particle cryo‑EM experience, ideally with SMC protein complexes, and a PhD in