Stage - master biologie moleculaire - h/f

Inserm France; Rennes; On-site; EUR 12,000 - 17,000; Full time

Job summary

Inserm France recherche un stagiaire Master 2 en biologie moléculaire pour un projet innovant sur les DNA scaffolds et la régulation spatiotemporelle des assemblages de kinases via des plateformes DNA origami. Vous contribuerez à l’expression et purification de la protéine N-terminale et au bioconjugage sur des origami ADN, avec validation par AFM et analyses enzymatiques.

Le stage se déroule à Rennes, en France, et accepte des profils Master 1–2 avec un fort intérêt pour la biologie chimique et

Qualifications

• Etudiants en Master 1 ou Master 2 biosciences.
• Profil avec forte appétence pour la biologie moléculaire et les méthodes de chimie biologique.
• Bonne connaissance des techniques de bioconjugation et d’expression/protéines.

Responsibilities

• Exprimer, purifier et fonctionnaliser la région protéique N-terminale par réactifs chiraux.
• Assembler et purifier des structures d’origami ADN.
• Bioconjuguer des protéines sur l’origami ADN par hybridation.
• Valider les nanostructures protéine-ADN par AFM et autres techniques.
• Évaluer l’activité enzymatique des protéines via des assays biochimiques.”],
• CoT_job_summary_short
• COMPANY NAME: Inserm France
• KEY POINTS: Stage Master 2 Biologie Moléculaire, DNA origami, AFM/CryoEM, purification et bioconjugation

Skills

Biologie moléculaire

Biochemie

Biophysique

Chimie biologique

Education

Master 1 ou Master 2 validé

Tools

AFM

CryoEM

DNA origami

Job description

STAGE MASTER 2 - BIOLOGIE MOLECULAIRE - H/F

The company

About the position

Internship in Chemical Biology

DNA scaffolds for tunable spatiotemporal control over receptor kinase assemblies.

Scientific background – Cellular stress induced by the abnormal accumulation of improperly folded proteins in the endoplasmic reticulum (ER) is emerging as a major actor in disease development and an appealing actionable target.[1] ER stress levels are under constant surveillance by the unfolded protein response (UPR), a major adaptive mechanism that lies at the core of cellular homeostasis and is responsible for cellular life-or-death decisions. The Inositol-requiring enzyme 1 (IRE1), the most evolutionary conserved UPR transducer, is an ER-resident transmembrane protein with a cytosolic dual kinase/RNase activity controlling pro-survival or pro-death signals. Yet, despite >20 years of investigations, the precise molecular mechanisms by which IRE1 is activated and exerts its catalytic and scaffolding functions still remain unclear and a subject of debate.[2] The group aims at deciphering the molecular mechanisms of IRE1 activation through a unique and novel prism at the interface of chemical biology, supramolecular chemistry and structural biology.

Description of the project & duties

This internship is in support of a PhD project focusing on using a programmable synthetic platform made of DNA structures (DNAorigami) to control the nanoscale organization and nature of different complexes of a kinase protein.[3]

As part of this internship, you will focus on:

1. Expressing, purifying and functionalizing the protein kinase N-terminus using a small molecule reagent.
2. Assembling and purifying DNA origami structures.
3. Bioconjugating proteins on the DNA origami using DNA hybridization principles.
4. Validating by AFM microscopy the functionalized Protein-DNA nanostructure obtained.
5. Evaluating the enzymatic activities of the protein using established biochemical assays and molecular biology techniques.

Main activities and techniques involved

• Molecular biology (design and conception of plasmids).
• Protein expression in eukaryotic cells.
• Protein engineering, bioconjugation, purification (affinity & SEC chromatography).
• Enzymatic assays.
• Structural characterization of protein-DNA conjugates (AFM, CryoEM).

Applicant profile

We encourage applications from highly motivated students from the national and international community with a background in chemical biology, biochemistry, biophysics, molecular biology, or other life sciences and with outstanding qualifications. Application only on this platform.

Application requirements

Required document(s)

Position characteristics

Offer info

Published on 10/08/2026 Contractuel Catégorie A Internship - 6 months Full time Beginner accepted License not required Second year to fourth year university level Master 1 validé

To be defined according to profile

04/01/2027