Postdoc: Cross-Kingdom RNAi in Plant-Bacteria

Cnrs Inc; France; On-site; EUR 42,000 - 54,000; Full time

Job summary

CNRS – Institut de biologie de l'Ecole Normale Supérieure seeks a First Stage Researcher to investigate how small RNAs direct post-transcriptional gene silencing in a phytopathogenic bacterium. The project combines molecular biology, proteomics and genetics within an international team.

The candidate will identify Arabidopsis factors taken up by Pseudomonas syringae during infection, and characterize RNA-binding proteins through biochemical and genetic approaches to illuminate AGS activity and

Qualifications

• Expertise is required in molecular biology and biochemistry.
• Genomics and cell biology techniques are essential.
• Experience with cloning, RT-qPCR, Western blot, and co-immunoprecipitation preferred.
• Experience with sequencing of small RNAs and mRNAs following protein IP.

Responsibilities

• Characterize mechanisms by which small RNAs direct post-transcriptional gene silencing in a phytopathogenic bacterium.
• Identify Arabidopsis factors taken up by a Pseudomonas syringae strain during infection via proteomic approaches.
• Use genetics and biochemical methods to study the function of RNA-binding proteins.
• Contribute to cross-kingdom RNAi research and gene regulation studies.

Skills

Molecular biology

Biochemistry

Genomics

Cell biology

Cloning

RT-qPCR

Tools

Western blot

Co-immunoprecipitation

Small RNA sequencing

mRNA sequencing

Northern blot

Job description

CNRS – Institut de biologie de l'Ecole Normale Supérieure seeks a First Stage Researcher to investigate how small RNAs direct post-transcriptional gene silencing in a phytopathogenic bacterium. The project combines molecular biology, proteomics and genetics within an international team.

The candidate will identify Arabidopsis factors taken up by Pseudomonas syringae during infection, and characterize RNA-binding proteins through biochemical and genetic approaches to illuminate AGS activity and