Immunologist Associate Scientist/Scientist

Alaya.bio; Villejuif; Sur place; EUR 40 000 - 60 000; Plein temps

Résumé du poste

A biotech company located in the Paris area is seeking a skilled professional to design and execute in vitro assays for gene delivery efficiency. The ideal candidate holds an MSc in Immunology or a related field, with strong expertise in flow cytometry and experience in CAR T-cell generation being a plus. This role offers the opportunity to work in a fast-paced environment with international exposure.

Qualifications

• Experience in ex vivo CAR T generation is a plus.
• Experience with animal studies is a plus.
• Proficiency in English is essential.

Responsabilités

• Design, plan and execute in vitro assays on primary cells.
• Perform multiparametric flow cytometry analyses.
• Write technical documents including SOPs and study reports.

Connaissances

Hands-on expertise in PBMCs isolation

Strong expertise in multiparametric flow cytometry

Ability to work in a fast-paced environment

Good command of IT and data analysis tools

Formation

MSc in Immunology & Cellular Biology or related field

PhD

Outils

FlowJo

GraphPad Prism

Description du poste

Overview

Alaya.bio is a preclinical-stage biotech company pioneering a new generation of polymeric nanoparticles applied to the rapid and universal in situ manufacturing of CAR T-cells. Founded in July 2022 and based in Paris and Cambridge, Alaya.bio is backed by industry leaders and investors with long track-records in cell therapy. The potential of our universal in situ CAR T technology has been recognized by the closing of five partnerships with renowned players in the clinical development of these groundbreaking therapies. The position is located in Paris area.

Key Responsibilities

• Design, plan and execute in vitro assays on primary cells for the characterization of the gene delivery efficiency of polymeric nanoparticles, in close collaboration with our Cell Biology Senior Scientist.
• Perform multiparametric flow cytometry analyses for in vitro and in vivo studies.
• Perform a variety of cellular assays & molecular assays (e.g. multiplex cytokine assays, cytotoxicity assays, ELISA, qPCR, preparation/purification of DNA and RNA).
• Culture human primary cells and cell lines (adherent and in suspension) for transient and stable transgene expression studies.
• Process and analyze animal blood and tissue samples (single cell suspension preparation from organs, plasma preparation, multiplex cytokine and enzyme assays, nucleic acid extraction and qPCR).
• Collect, analyze and report data using appropriate software including electronic lab notebook.
• Support day-to-day laboratory operations, including reagents and consumables purchasing, maintenance of equipment, management of biological, infectious & care activity wastes.
• Compliance with regulatory standards and H&S rules in place in the company.
• Writing of technical documents including SOPs, experimental protocols and study reports.

Your Profile

• MSc in Immunology & Cellular Biology or related field; PhD is a plus.
• Hands-on expertise in PBMCs isolation, bead-based enrichment, culture of primary cells and cellular assays.
• Strong expertise in multiparametric flow cytometry.
• Experience in ex vivo CAR T generation (including large-scale processes) and characterization is a plus.
• Experience with animal studies is a plus.
• Ability to work in a fast-paced environment, meet deadlines, and prioritize work on multiple projects.
• Proficiency in English (written and spoken) is essential, given the international exposure of the company.
• Good command of IT and data analysis tools (FlowJo, GraphPad Prism).