Doctorant_076_mk - h/f

Inserm France; Illkirch-Graffenstaden; On-site; EUR 32,000 - 40,000; Full time

Job summary

Inserm France invites applications for a fixed-term research position focused on fundamental skeletal muscle biology. The project will examine molecular mechanisms controlling local protein synthesis at the myotendinous junction and perform transcriptomic characterization of muscle cell maturation in vitro.

Activities include generating mouse mutants, analyzing the local translatome, performing biochemical identifications using affinity-tagged UTRs, and presenting results at meetings and

Qualifications

• Master degree in a relevant field or equivalent.
• Experience with mouse genetics and phenotyping.
• Experience with histology and imaging techniques.
• Familiarity with proteomics/NGS data is a plus.

Responsibilities

• Genotype mice and analyze phenotypes.
• Study local translatome changes upon gene perturbations.
• Perform biochemical pull-downs and identify interacting proteins.
• Present findings in lab meetings and conferences.
• Supervise Master students.

Skills

Mouse genetics

Histological analyses

Imaging

Physiological tests

Mass-spectrometry

NGS

Communication

Team collaboration

Education

Master's degree

Tools

Mass-spectrometry

NGS

Job description

About the position

Research will be in the field of fundamental skeletal muscle biology and will consist of two complementary scientific axes:(1) investigation of the molecular mechanisms controlling local protein synthesis at the muscle-tendon junction, and (2) transcriptomic characterization of the maturation of skeletal muscle cells generated in vitro.

Desired profile

Main Activities

Myotendinous junction (MTJ) is a mechanical buffer of the skeletal muscle cell and prone site to injury upon exercise. Our lab recently developed a new tool to manipulate the MTJ domain and revealed its local translational program as well as how it rewires after exercise. However, the molecular mechanism by which this special translation program is achieved remains unknown. The student will take both candidate and unbiased approaches to address this problem.

• Use mouse genetics to generate mutants of candidate RNA binding proteins and dissect their phenotype.
• Investigate the local translatome upon mutating RNA binding proteins and identify their binding partners.
• Biochemical pull-down identification of candidate factors using affinity tag fused UTRs.

Associated Activities

• Presentation of the work in lab meetings and conferences. Supervising Master students.

Specific Requirements or Constraints

• Mouse genetics skills: maintenance, genotyping, histological analyses, and imaging
• Physiological tests: various assays for phenotyping muscle function
• Biochemical skills for mass-spectrometry, NGS etc.

Skills/Qualifications

• Communication skills (presentation, writing)
• Collaborative team spirit

Application requirements

Required document(s)

Position characteristics

Offer info

Published on 10/21/2026 Contractuel Catégorie A Fixed-term contract - 36 months Full time Beginner accepted License not required Master degree level MASTER