Cryo-EM Membrane Protein Scientist (Insect/Mammalian)

Cnrs Inc; France; Presencial; EUR 36.000 - 54.000; Jornada completa

Descripción de la vacante

The Institute of Structural Biology (IBS) at CNRS in Grenoble invites applications for a researcher to conduct cell biology experiments using insect and mammalian cells, and to advance expression, solubilisation, purification and structural analysis of membrane proteins. The role involves close collaboration with EM facilities at IBS and partner institutes.

The successful candidate will work within the Membrane Group in the Moreau team, contributing to cryo-EM data collection, image processing

Formación

• Expertise in protein production in insect and mammalian cells.
• Expertise in purification of membrane proteins.
• Expertise in biochemical and biophysical techniques for protein characterisation.
• Expertise in cryo-electron microscopy and crystallography techniques.
• Knowledge of bioinformatics tools for analysing cryo-electron microscopy images.
• Knowledge of molecular biology techniques (PCR, DNA purification).

Responsabilidades

• Carry out the production of membrane proteins in insect and mammalian cells using baculoviruses or lentiviruses.
• Use, develop and adapt protocols for the solubilisation, purification and concentration of membrane proteins.
• Carry out, whilst adapting the experimental conditions, a range of biochemical and biophysical techniques for the quality control of samples.
• Assist with the preparation and analysis of samples for cryo-electron microscopy.
• Analyse cryo-electron microscopy images to reconstruct the three-dimensional structure of membrane proteins in their apo and holo forms.
• Interpret and present the results of the analyses, ensuring their follow-up and quality
• Draft experimental reports and protocols
• Manage and organise technical resources and biological materials in coordination with partner platforms and laboratories
• Keep track of scientific and technological developments within their field of activity
• Contribute to the dissemination and promotion of results through oral presentations and publications

Conocimientos

Protein production

Membrane protein purification

Biochemical techniques

Biophysical techniques

Cryo-EM

Crystallography

Bioinformatics

Molecular biology

Collaboration

Scientific reporting

Descripción del empleo

The Institute of Structural Biology (IBS) at CNRS in Grenoble invites applications for a researcher to conduct cell biology experiments using insect and mammalian cells, and to advance expression, solubilisation, purification and structural analysis of membrane proteins. The role involves close collaboration with EM facilities at IBS and partner institutes.

The successful candidate will work within the Membrane Group in the Moreau team, contributing to cryo-EM data collection, image processing