PhD student (m/f/d)

Karriereamukb; Bonn; On-site; EUR 39,000 - 55,000; Part time

Benefits offered by this job

TV-L EG13 pay

Annual bonus

Company pension

Flexible working time models

Daycare center access

Good public transport access

Training opportunities

Structured onboarding

Health and wellness programs

Employee discount programs

Job summary

University Hospital Bonn, Institute of Biochemistry and Molecular Biology, invites applications for a PhD student (m/f/d) in Bonn. The position (25.025 hours/week) is a three-year term with potential extension.

The project investigates molecular mechanisms coordinating protein import with inter-organellar contacts and metabolism using advanced biochemistry, molecular biology and proteomics approaches. The team applies robot-based genetic screens, fluorescence imaging and proteomic assays to

Qualifications

• Diploma or master’s degree in Biology, Biochemistry, Molecular Medicine, Chemistry or related subject.
• Experience with biochemical, cell and molecular biology techniques or proteomics is advantageous.
• Highly motivated, team-oriented candidate with interest in protein biochemistry.

Responsibilities

• Study molecular mechanisms coordinating protein import with inter-organellar contacts and metabolism.
• Perform robot-based genetic screens, fluorescent imaging, protein–protein interaction studies and proteomic assays.
• Reconstitute mitochondrial protein import and assembly in cell-free systems and in vitro.

Skills

Biochemical techniques

Molecular biology techniques

Proteomics

Education

Diploma or Master’s in Biology/Biochemistry/Molecular Medicine/Chemistry

Job description

PhD student (m/f/d)

ST-54074

The following part-time (25,025 hours/week) is to be filled as soon as possible in the Institute of Biochemistry and Molecular Biology of the University Hospital Bonn:

PhD student (m/f/d)

The position is limited until December 31, 2030.

Mitochondria fulfil various essential functions for the survival of eukaryotic cells. Their biogenesis and functions depend on the import of more than 1000 different proteins that are produced as precursors on cytosolic ribosomes. The translocase of the outer membrane (TOM complex) forms the general entry gate. Subsequently, specific protein translocases import these proteins into the mitochondrial subcompartments. We identified a plethora of interaction partners of mitochondrial protein translocases, linking them to protein complex assembly, organellar contact sites, energy metabolism and quality control (Ellenrieder et al., 2019; Mårtensson et al., 2019, Song et al., 2023). In this project, we will study the molecular mechanisms that coordinate protein import with inter-organellar contacts and metabolism.

We use highly innovative techniques in biochemistry, molecular and cell biology. This includes robot based genetic screens combined with fluorescent imaging and protein-protein-interaction studies coupled with proteomic assays. We study the functionality and fate of proteins using protein stability, aggregation, disaggregation assays and analyse the state of post-translational modifications with ubiquitin-family proteins. Moreover, we reconstitute the import and assembly of mitochondrial proteins synthesized in cell-free systems into intact organelles in vitro. We offer a well-equipped workspace in an active international team. The University of Bonn provides an excellent scientific research environment.

The position starts as soon as possible and is for a period of three years, with the possibility of an extension.

In this project, we will study the molecular mechanisms that coordinate protein import with inter-organellar contacts and metabolism.

We use highly innovative techniques in biochemistry, molecular and cell biology. This includes robot based genetic screens combined with fluorescent imaging and protein-protein-interaction studies coupled with proteomic assays. We study the functionality and fate of proteins using protein stability, aggregation, disaggregation assays and analyse the state of post-translational modifications with ubiquitin-family proteins. Moreover, we reconstitute the import and assembly of mitochondrial proteins synthesized in cell-free systems into intact organelles in vitro. We offer a well-equipped workspace in an active international team. The University of Bonn provides an excellent scientific research environment.

Ihr Profil

The ideal candidate will be a highly motivated, team-oriented graduate with a strong interest in protein biochemistry, molecular and cell biology. Experience with biochemical, cell and molecular biology techniques or proteomics is advantageous. Candidates should hold a diploma or a master's degree in Biology, Biochemistry, Molecular Medicine, Chemistry or a related subject.

• Responsible and diverse: A position with plenty of room for creativity and responsibility within a supportive and collegial team
• Fair compensation: Pay according to the TV-L (EG13)collective agreement, including annual bonus and company pension scheme
• Family-friendly flexibility: Flexible working time models, access to a place in our company daycare center, and programs supporting employees returning from parental leave
• Smart commuting: Excellent connection to the SWB public transport network
• Supported development: Individual training opportunities through courses, e-learning programs, and coaching sessions
• Structured onboarding: A systematic introduction to your new role and team
• Healthy at work: A wide range of health and wellness programs, plus free membership at the UKB fitness studio
• Employee benefits: Access to discounted offers for employees (corporate benefits)