Lab Technician for In Vivo Multiplexed CRISPR Screening

VIB; Oost-Vlaanderen; Sur place; EUR 42 000 - 56 000; Plein temps

Avantages offerts par ce poste

Hospitalization insurance

Pension plan

Eco-cheques

Bicycle allowance

Public transport reimbursement

Generous annual leave policy

Résumé du poste

VIB's Laboratory of Causal Systems Immunology seeks a wet-lab technician to support in vivo CRISPR screening. You will join the PowerPortrait ERC project, culture primary murine stem cells, and genetically modify them to generate transgenic mice.

Expect cell/mouse work, transduction, flow cytometry, microscopy, irradiation, and routine buffer prep in a high-tech, collaborative environment. You will work under staff scientists, with training opportunities and access to core facilities, within a

Qualifications

• Bachelor's degree or higher in biomedical laboratory technology, biochemistry, biotechnology, animal care or a related field.
• FELASA B/C certificate or willingness to obtain the certificate promptly.
• Fluency in written and spoken English.
• Experience with in vitro work and mouse handling.
• Experience with molecular techniques (PCR, qPCR).
• Knowledge of lab health and safety procedures.
• Previous lab experience; team player.

Responsabilités

• Support in vivo CRISPR screening within the ERC PowerPortrait project.
• Culture primary murine stem cells in vitro and genetically modify them.
• Perform cell work, mouse work, transduction, and flow cytometry (FACS).
• Operate multicolor flow cytometry, microscopy and irradiation.
• Assist with buffer preparation and general lab tasks.

Connaissances

In vitro work

Mice handling

PCR

qPCR

Laboratory safety

Teamwork

English proficiency

Formation

Bachelor's degree or higher in biomedical lab field

Description du poste

About Us

The Laboratory of Causal Systems Immunology combines large-scale in vivo perturbation experiments, advanced mouse models of immune diseases and causal learning to uncover how genes shape immune-cell states and predict how the immune system responds to interventions. This tight integration of experimental immunology with data-driven experiments allows us to tackle fundamental biological questions with direct biomedical relevance. We are embedded in both the VIB-UGent Center for Inflammation Research, a leading center unravelling the cellular and molecular mechanisms of inflammatory diseases, and the VIB Center for AI & Computational Biology, a young, ambitious center whose research groups address biological questions through the lens of AI.

The Laboratory of Causal Systems Immunology combines large-scale in vivo perturbation experiments, advanced mouse models of immune diseases and causal learning to uncover how genes shape immune-cell states and predict how the immune system responds to interventions. This tight integration of experimental immunology with data-driven experiments allows us to tackle fundamental biological questions with direct biomedical relevance. We are embedded in both the VIB-UGent Center for Inflammation Research, a leading center unravelling the cellular and molecular mechanisms of inflammatory diseases, and the VIB Center for AI & Computational Biology, a young, ambitious center whose research groups address biological questions through the lens of AI.

About The Role

The lab is looking for a wet-lab technician to support in vivo CRISPR screening. You will join the ERC Starting Grant project PowerPortrait, which aims to disentangle the regulation of a key immune cell in health and disease. Under the supervision of a staff scientist, you will work both collaboratively and independently on CRISPR screens from start to finish using various high-end technologies. This involves the in vitro culture of primary murine stem cells and genetically altering them using CRISPR technology to generate transgenic mice. You will perform a variety of techniques, including cell and mouse work, cell transduction, multicolor flow cytometry and FACS sorting, light/fluorescence and confocal microscopy and irradiation. In addition, you will support general tasks within the lab such as preparing buffers. We offer a high-tech environment, support from several core facilities, training opportunities and a diverse, vibrant team.

Essential Requirements

• A Bachelor's degree or higher in biomedical laboratory technology, biochemistry, biotechnology, animal care or a related field


• FELASA B/C certificate or willingness to obtain the certificate in a timely manner


• Fluency in written and spoken English


• Experience with in vitro work


• Experience working with mice


• Experience with molecular techniques such as PCR and qPCR


• Knowledge of laboratory health and safety procedures


• Previous laboratory experience (placements completed during degree studies are sufficient)


• Ability to work effectively as part of a team

Desirable Bot Not Required

• Experience in culturing stem cells


• Experience with intravenous and intraperitoneal injections.


• Experience with cell transfection techniques (particularly viral transduction).


• Basic understanding of immunology


• Basic understanding of flow cytometry and confocal microscopy

Key personal characteristics

• You are enthusiastic and curious about scientific research


• You like learning new techniques and want to stay at the technological forefront


• You are willing to participate in the general lab organization


• You can work well in a team and independently


• You are accurate and critical of your work and its implementation


• You have a strong ability to multitask and meet deadlines


• You enjoy other cultures and are respectful of others and their different ideas and opinions

Our offer

• A full-time position


• A challenging job in a stimulating, collaborative environment with opportunities for personal development


• You will learn new techniques and tools


• Freedom to shape your work and role


• Exposure to diverse projects and cutting-edge technologies


• A generous annual leave policy to create a good work-life balance


• A competitive salary and benefits including hospitalization insurance, pension plan, eco-cheques, a bicycle allowance or full reimbursement of public transport costs


• This role is part of a 5-year project, with the possibility of extension depending on the availability of funds.

In case of questions, please reach out to [e-mail].