Technician B - Cardiology

Columbia University; New York, Northern (NY, KY); Hybrid; USD 51,000 - 62,000; Full time

Job summary

Columbia University is seeking a Technician B to provide bench and data analysis support across cell biology, immunology, and functional genomic assays. The role contributes to experiments and general research activities within a dynamic lab team.

The position involves maintaining primary mouse and human cell cultures, performing molecular assays, and assisting with animal models, sequencing experiments, data interpretation, and manuscript preparation.

Qualifications

• Bachelor's degree in biology, chemistry, biochemistry, molecular biology or related field.
• Experience in a research lab preferred and able to work with animal models.
• Comfort with experimental design, data analysis and interpretation.

Responsibilities

• Ensure compliance with study protocols (IACUC/IRB) and maintain lab safety.
• Perform routine cellular and molecular assays (transformation, RNA/DNA prep, cDNA synthesis, qRT-PCR, transfection, ELISA, Western blot).
• Maintain mouse and human cell cultures, including iPSC lines and differentiation.
• Use microscopy and FACS for cell characterization and functional assays.
• Prepare histology sections, staining, imaging and data analysis.
• Assist with breeding, genotyping and animal model preparation.
• Support RNA-seq, single-cell RNA-seq, CRISPR screens and high-content imaging.
• Interpret and present data; contribute to manuscript prep.
• Order and manage lab supplies and equipment.
• Perform other duties as needed.

Skills

Laboratory skills

Data analysis

Communication

Teamwork

Education

Bachelor's degree in biology or related field

Tools

FACS

qRT-PCR

ELISA

Western Blot

Job description

• Job Type: Support Staff - Union
• Bargaining Unit: SSA
• Regular/Temporary: Regular
• Hours Per Week: 35
• Salary Range: $56,489.86 to $56,489.86

The salary of the finalist selected for this role will be set based on a variety of factors, including but not limited to departmental budgets, qualifications, experience, education, licenses, specialty, and training. The above hiring range represents the University’s good faith and reasonable estimate of the range of possible compensation at the time of posting.

Position Summary

Technician B will provide bench and data analysis support for cell biology, molecular biology, immunology, and functional genomic assays, and assist the lab team with experiments and general research activities.

Responsibilities

• Will be responsible for assuring compliance with study protocols, including IACUC and IRB, and laboratory safety.
• Will perform routine cellular and molecular assays (e.g., transformation, miniprep, maxiprep, RNA, DNA and protein preparation, cDNA synthesis, qRT-PCR, transfection, packaging of viral vectors, ELISA, Western Blotting, etc.).
• Will maintain primary mouse and human cell culture and human induced pluripotent stem cell culture and differentiation.
• Will utilize microscopic techniques and FACS for characterization/functional assays of cell lines and primary cells.
• Will perform histological sectioning, staining, imaging, and quantitative data analysis.
• Assist with other lab team efforts, including breeding, genotyping, and preparation of animal models for experiments and surgeries.
• Assist with sample preparation of RNA‑seq, single cell RNA‑seq, CRIPSR screening, high content imaging, and other functional genomic assays.
• Interpreting and presenting data, and participating in manuscript preparation.
• Ordering and maintaining laboratory supplies, chemicals, and equipment.
• Performing other duties as required.

Minimum Qualifications

• A Bachelor’s degree in biology, chemistry, biochemistry, molecular biology, biostatistics, or other biomedical research related fields or equivalent in education training and experience.

Preferred Qualifications

• Experiences in a research laboratory
• Familiar with cell biology, molecular biology, immunology, and functional genomic techniques, and animal models
• Capable of developing skills in experimental design, data analysis, visualization, and interpretation.

Other Requirements

• Good organizational and communication skills.
• Ability to work well as part of a team, as well as independently.
• The candidate selected for this position is required to undergo a drug screening test prior to a final offer of employment being made.
• Must successfully complete systems training requirements.
• Participation in the medical surveillance program
  • Work with laboratory animals
  • Potential bloodborne pathogen exposure

Equal Opportunity Employer / Disability / Veteran

Columbia University is committed to the hiring of qualified local residents.