Targeting RBCK1 disease mechanisms

Italian Ministry of Education, University and Research; Town of Italy (NY); On-site; USD 34,000 - 45,000; Full time

Job summary

ALMA MATER STUDIORUM - UNIVERSITA' DI BOLOGNA - - DIPARTIMENTO DI SCIENZE MEDICHE E CHIRURGICHE seeks a research fellow to design experiments and work under supervision to differentiate patient-derived iPSCs into 2D musculoskeletal cells and create 3D organoid models. The role includes inflammation profiling, transcriptomics, and drug testing for potential therapies.

The fellow will also pursue gene therapy approaches using CRISPR/Cas9, contributing to cutting-edge translational research in a

Qualifications

• Eligibility of fellows: country/ies of residence: All

Responsibilities

• Develop the experimental plan under supervision of the tutor and a senior postdoc.
• Differentiate iPSC lines into 2D musculoskeletal stem cells and generate 3D organoid models.
• Characterise inflammatory profile, perform molecular genetics and transcriptomics under basal conditions and after LPS stress.
• Induce mitochondrial dysfunction with galactose and analyse results.
• Test FDA/EMA-approved drugs to identify effective therapy.
• Develop gene therapy using CRISPR/Cas9 techniques.

Job description

ALMA MATER STUDIORUM - UNIVERSITA' DI BOLOGNA - - DIPARTIMENTO DI SCIENZE MEDICHE E CHIRURGICHE

Organisation/Company ALMA MATER STUDIORUM - UNIVERSITA' DI BOLOGNA - - DIPARTIMENTO DI SCIENZE MEDICHE E CHIRURGICHE Research Field Medical sciences » Medicine Researcher Profile Recognised Researcher (R2) Leading Researcher (R4) First Stage Researcher (R1) Established Researcher (R3) Application Deadline 31 Oct 2026 - 23:59 (UTC) Country Italy Type of Contract To be defined Job Status Not Applicable Is the job funded through the EU Research Framework Programme? Not funded by a EU programme Is the Job related to staff position within a Research Infrastructure? No

Offer Description

The research fellow will develop the experimental plan under the supervision of the tutor and a senior postdoc. Starting with iPSC lines previously derived from patients' fibroblasts, the researcher will be responsible for differentiating them into 2D musculoskeletal stem cells and for generating 3D organoid models. To characterise the inflammatory profile, molecular genetics and transcriptomics experiments will be conducted under basal conditions and following LPS-induced stress. For mitochondrial dysfunction, the system will be stressed instead with galactose and similarly analysed. Once a reliable readout has been identified from the stem cell characterisation experiments, a panel of FDA/EMA-approved drugs will be tested to identify an effective therapy. In addition, the researcher will conduct experiments to develop gene therapy using genetic engineering techniques such as CRISPR/Cas9.

• Italy

Eligibility of fellows: country/ies of residence:

• All

Eligibility of fellows: nationality/ies:

Number of offers available 1 Company/Institute ALMA MATER STUDIORUM - UNIVERSITA' DI BOLOGNA - - DIPARTIMENTO DI SCIENZE MEDICHE E CHIRURGICHE Country Italy