T-Cell Discovery Scientist – High-Throughput Immunology

Dawar Consulting, Inc.; San Diego (CA); On-site; USD 58,000 - 79,000; Full time

Benefits offered by this job

Medical

Paid Sick leave

401K

Job summary

Dawar Consulting, Inc. in San Diego, CA is seeking a Scientist – Discovery Biology/ T cells to support a high-priority binder-screening campaign. You will characterize candidates (scFvs/VHHs) for potency, specificity, affinity, functionality, and safety through T-cell engineering and functional validation assays.

The role requires 2+ years of wet-lab experience, expertise in flow cytometry, and non-viral transfection. This is a long-term contract with benefits and on-site work in San Diego.

Qualifications

• Bachelor's or Master's degree in Immunology, Molecular Biology, Bioengineering, or a related biological science.
• Minimum 2 years of hands-on wet-lab experience, preferably in an industry setting.
• Strong aseptic technique and experience isolating primary T cells from PBMCs.
• Mandatory expertise in flow cytometry acquisition and data analysis.

Responsibilities

• Perform non-viral engineering of primary human T cells using mRNA or transposon-based DNA systems via electroporation/nucleofection; monitor cell viability, recovery, and receptor expression.
• Isolate, maintain, and expand primary T cells from PBMCs and Jurkat cell lines using aseptic techniques.
• Execute high-throughput binder screening and multi-color flow cytometry (FACS) to assess surface expression and phenotypic markers; analyze data using FlowJo.
• Conduct T-cell activation and functional assays, including cytotoxicity assays, cytokine profiling, and proliferation assays.
• Record protocols, deviations, raw data, and experimental results in an ELN to support data integrity and regulatory filings.
• Support screening scale-up using liquid-handling robotics, as applicable.

Skills

Wet-lab experience

Aseptic technique

Flow cytometry data analysis

Non-viral transfection/electroporation

Education

Bachelor's or Master's degree in Immunology, Molecular Biology, Bioengineering, or related biological science

Tools

FlowJo

Electronic Lab Notebook (ELN)

Job description

Dawar Consulting, Inc. in San Diego, CA is seeking a Scientist – Discovery Biology/ T cells to support a high-priority binder-screening campaign. You will characterize candidates (scFvs/VHHs) for potency, specificity, affinity, functionality, and safety through T-cell engineering and functional validation assays.

The role requires 2+ years of wet-lab experience, expertise in flow cytometry, and non-viral transfection. This is a long-term contract with benefits and on-site work in San Diego.