Stem Cell & Skeletal Biology Research Assistant

University of California - Davis; Davis (CA); Presencial; USD 66 000 - 73 000; Tempo integral

Resumo da oferta

The Fierro lab at UC Davis, within the Department of Cell Biology and Human Anatomy, School of Medicine, seeks an Assistant Specialist (full- or part-time) to advance interdisciplinary stem cell and skeletal biology studies. The role includes data analysis, methods development, and collaboration with physicians in orthopedic research.

Responsibilities cover experimental design, supervision of students, and coordination of projects, with emphasis on microCT, ELISA, flow cytometry, and molecular

Qualificações

• Experience in RNA extraction, cDNA synthesis and RT-PCR, protein extraction and Western Blots, immunohistochemistry, immunocytochemistry, ELISA, mouse surgery and dissection, cloning, viral packaging and transduction, flow cytometry, and culture of both primary cells and immortalized cell lines.
• Evidence of academic accomplishment such as referred journal papers, conference presentations, and grant writing.
• Experience mentoring other lab members.
• Evidence of academic accomplishment such as referred journal papers and conference presentations.

Responsabilidades

• Handle mouse breeding, induce recombination by tamoxifen administration, collect tissue and analyze collected bones by microCT and immunohistochemistry and biomarkers in sera by ELISA.
• Perform culture of mouse and human MSCs. Overexpress and silence genes using lentiviral vectors, and perform analysis of gene expression, protein levels, and osteogenic markers such as measurements of alkaline phosphatase activity and calcium phosphate precipitation.

Conhecimentos

RNA extraction

RT-PCR

Western Blots

Immunohistochemistry

Immunocytochemistry

ELISA

Mouse surgery

Dissection

Cell culture

Viral transduction

Cloning

Flow cytometry

Formação académica

Bachelor's degree + 3+ years research experience

Master's degree in biology

Ferramentas

microCT

Lentiviral packaging

Recombinant DNA techniques

Tamoxifen induction

Descrição da oferta de emprego

The Fierro lab at UC Davis, within the Department of Cell Biology and Human Anatomy, School of Medicine, seeks an Assistant Specialist (full- or part-time) to advance interdisciplinary stem cell and skeletal biology studies. The role includes data analysis, methods development, and collaboration with physicians in orthopedic research.

Responsibilities cover experimental design, supervision of students, and coordination of projects, with emphasis on microCT, ELISA, flow cytometry, and molecular