Sr. Research Associate- ESI Flow Cytometry

Yoh Services LLC; Waltham (MA); On-site; USD 38,572 - 55,104; Full time

Benefits offered by this job

Medical, Prescription, Dental & Vision Benefits

Health Savings Account (HSA)

Life & Disability Insurance

Employee Assistance Program (EAP)

401K Retirement Savings Plan

Certification and training opportunities

Job summary

Yoh Services LLC is seeking a Research Associate in Waltham, MA, to apply expertise in flow cytometry and support the vaccine R&D function. The role requires experience in lab-based tasks, including multi-parameter flow cytometry and data analysis. Applicants should possess a relevant degree with practical experience. The job offers a range of benefits including medical coverage, wellness programs, and retirement plans, ensuring support for employees beyond their work responsibilities.

Qualifications

• Knowledge of immunoassay instruments including flow cytometers and plate readers.
• Hands-on expertise with multi-parameter flow cytometry (minimum 10-color panels).
• Demonstrated experience with intracellular cytokine staining in preclinical and/or human PBMC samples.

Responsibilities

• Support experimental assay design and execute multi-parameter flow cytometry panels.
• Optimize sort gates and collection strategies to maximize cell recovery and purity.
• Analyze flow cytometry data using industry-standard software.

Skills

Proficiency in flow cytometry data analysis software (OMIQ preferred)

Ability to express oneself clearly and concisely within team

Good time management and prioritization skills

Attention to detail

Education

BS with 4+ years or MS with 2+ years relevant experience

Degree in Immunology, Biotechnology, Virology, Biochemistry or related field

Tools

Flow cytometer analyzers

Intracellular Cytokine Staining assays

Data analysis software (OMIQ)

Job description

Research Associate - ESI Flow Cytometry

The Yoh resource is expected to apply expertise across several practical skills including flow cytometry and cell sorting in support of the Client’s Vaccines R&D Global Immunology function. The role is a lab-based research scientist who will be participating in various teams to support preclinical and early clinical evaluation of vaccine candidates against various respiratory viruses. Knowledge of immunoassay instruments, including flow cytometer analyzers, high-speed sorter, centrifuges, multi-mode plate readers and plate washers will be required. The individual will be performing laboratory work including flow cytometry and immunoassays, recording data in specified formats and/or recording detailed day-to-day work in electronic laboratory notebook. Responsibilities will include reagent preparation, lab housekeeping and flow cytometry analyzers and cell sorters maintenance. Applicant must be capable of working both independently and as part of a structured team.

Responsibilities

• Support experimental assay design, optimization, and execute multi-parameter flow cytometry panels (beyond 20 colors) for comprehensive T cell and B cell subset identification
• Execute multi-cytokine Intracellular Cytokine Staining (ICS) assays (IFN-γ, TNF-α, IL-2, IL-4, IL-17, etc.) following antigen-specific stimulation. Operate and maintain flow cytometers (conventional and spectral platforms) with adherence to quality control protocols
• Execute high-speed, high-purity cell sorting using Sony or similar instrumentation for isolation of antigen-specific T cell and B cell populations or other cell subsets of interest.
• Optimize sort gates and collection strategies to maximize cell recovery and purity
• Troubleshoot sort performance issues including clogging, deflection plate alignment, and collection efficiency
• Analyze flow cytometry data using industry-standard software (OMIQ)
• Apply appropriate gating strategies following established SOPs and scientific best practices
• Analyze and summarize experimental outcomes, record experimental procedures and raw and analyzed data in electronic laboratory notebook.
• Conduct experiments and prepare PowerPoint slides for internal presentations as required.
• Write standard operating procedures (SOPs), and documents for methods, instruments, or work processes as required.
• Collaborate as needed with internal partners in other functional areas, external vendors, and external scientific partners.
• Comply with the company safety policies and minimize risk to self, others, and the environment, consistent with the community and industry standards.

Education and Experience Requirements

• BS with 4+ years or MS with 2+ years relevant experience. Degree should be in Immunology, Biotechnology, Virology, Biochemistry or related field.
• Hands‑on expertise with multi-parameter flow cytometry (minimum 10‑color panels) and/or cell sorting.
• Demonstrated experience with intracellular cytokine staining in preclinical and/or human PBMC samples and/or ability to execute sterile sorts, optimize sort gates, and troubleshoot sorter performance.

Key Skills, Abilities, and Competencies

• Proficiency in flow cytometry data analysis software (OMIQ preferred)
• Working knowledge with Good Research Practice (GRP).
• Ability to express oneself clearly and concisely within team.
• Good time management and prioritization skills.
• Ability to work in agile team environment.
• Attention to detail.
• Data review
• Training
• Scheduling
• GDP
• GRP, compliance requirements for clinical trial samples.

Estimated Minimum Rate: $28.00

Estimated Maximum Rate: $40.00

What’s In It for You?

Benefit eligibility is in accordance with applicable laws and client requirements. Benefits include:

• Medical, Prescription, Dental & Vision Benefits (for employees working 20+ hours per week)
• Health Savings Account (HSA) (for employees working 20+ hours per week)
• Life & Disability Insurance (for employees working 20+ hours per week)
• MetLife Voluntary Benefits
• Employee Assistance Program (EAP)
• 401K Retirement Savings Plan
• Direct Deposit & weekly e‑payroll
• Referral Bonus Programs
• Certification and training opportunities

Note: Any pay ranges displayed are estimations. Actual pay is determined by an applicant’s experience, technical expertise, and other qualifications as listed in the job description. All qualified applicants are welcome to apply.

Yoh, a Day & Zimmermann company, is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.

Visit https://www.yoh.com/applicants-with-disabilities to contact us if you are an individual with a disability and require accommodation in the application process.

For California applicants, qualified applicants with arrest or conviction records will be considered for employment in accordance with the Los Angeles County Fair Chance Ordinance for Employers and the California Fair Chance Act. All of the material job duties described in this posting are job duties for which a criminal history may have a direct, adverse, and negative relationship potentially resulting in the withdrawal of a conditional offer of employment.

It is unlawful in Massachusetts to require or administer a lie detector test as a condition of employment or continued employment. An employer who violates this law shall be subject to criminal penalties and civil liability.

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