Senior Scientist, Immunology - Targeted LNP Delivery

Tr1X; San Diego (CA); On-site; USD 152,000 - 165,000; Full time

Job summary

Tr1X is a clinical-stage biotechnology company developing engineered Tr1 cell therapies to restore immune balance in autoimmune and inflammatory diseases, including CAR‑Tr1 approaches. The Senior Scientist, In vivo Immunology – Targeted LNP Delivery, leads design and evaluation of tLNP formulations and immunological targeting strategies in vitro and in vivo, reporting to Biology leadership.

This hands-on role requires deep immunology expertise, strong data analysis, and collaboration across

Qualifications

• Ph.D. in Immunology or closely related field with 4+ years of hands-on immunology research experience.
• Experience applying targeted lipid nanoparticles (tLNPs) for cell-specific delivery to immune cells.
• Strong understanding of lymphocyte biology and in vivo disease models.
• Excellent communication, organization, and ability to work independently in a startup environment.

Responsibilities

• Design and lead in vitro and in vivo studies evaluating tLNP targeting and payload delivery.
• Collaborate with biology, pharmacology, and process development to advance candidate formulations.
• Apply flow cytometry and immunophenotyping to characterize tLNP uptake and functional readouts.
• Prepare technical reports and present findings to stakeholders.
• Stay current with immune cell-targeted LNP delivery and nucleic acid therapeutics.

Education

PhD in Immunology

Job description

About Tr1X

Tr1X is a clinical-stage biotechnology company developing engineered Tr1 cell therapies to restore immune balance in autoimmune and inflammatory diseases. Leveraging the unique biology of Tr1 cells, the company is advancing a pipeline of off-the-shelf allogeneic and in vivo CAR-Tr1 therapies designed to deliver targeted immunoregulation and address diseases driven by immune dysfunction, including transplant-related complications. Founded by pioneers in the discovery of Tr1 cells, Tr1X, Inc. is focused on developing durable, potentially curative therapies for conditions with high unmet need. For more information, visit www.tr1x.bio.

About Tr1X

Tr1X is a clinical-stage biotechnology company developing engineered Tr1 cell therapies to restore immune balance in autoimmune and inflammatory diseases. Leveraging the unique biology of Tr1 cells, the company is advancing a pipeline of off-the-shelf allogeneic and in vivo CAR-Tr1 therapies designed to deliver targeted immunoregulation and address diseases driven by immune dysfunction, including transplant-related complications. Founded by pioneers in the discovery of Tr1 cells, Tr1X, Inc. is focused on developing durable, potentially curative therapies for conditions with high unmet need. For more information, visit www.tr1x.bio.

Position Summary

The Senior Scientist, In vivo Immunology – Targeted LNP Delivery is an experienced, hands‑on immunologist who leads the biological design and evaluation of targeted lipid nanoparticle (tLNP) formulations supporting the Tr1X in vivo chimeric antigen receptor (CAR)-Treg platform. Reporting to the Executive Director of Biology, this individual plays a central R&D role in advancing tLNP delivery systems — defining the immunological targeting rationale and driving in vitro and in vivo proof‑of‑concept studies — to enable precise, immune cell‑specific delivery of nucleic acid payloads.

This is a high‑impact, science‑driven role focused on the discovery and development of Tr1X's next‑generation tLNP delivery platform. The Senior Scientist brings deep expertise in immunology, particularly T cell and Treg biology, hands‑on experience applying tLNPs in immune cells, strong experimental rigor across in vitro and in vivo studies, and the ability to thrive in a fast‑paced startup environment.

Key Responsibilities

• Partner with formulation and chemistry colleagues on the design and optimization of targeted lipid nanoparticle (tLNP) formulations for mRNA payloads, owning the immunological targeting criteria and biological evaluation.
• Design and execute in vitro and in vivo studies, including humanized models, to evaluate tLNP targeting specificity to defined immune cell subsets, biodistribution, potency, and functional delivery of payload within the Tr1X in vivo CAR‑Treg platform.
• Design and apply multiparameter flow cytometry and immunophenotyping assays to characterize tLNP uptake across immune cell subsets, targeting specificity, and downstream functional readouts such as transgene expression and suppressive activity.
• Develop and apply analytical characterization methods (e.g., DLS, encapsulation efficiency, fluorescence‑based assays) as needed to support formulation and conjugation research.
• Interpret analytical, in vitro, and in vivo data to establish structure‑function relationships between tLNP formulation/conjugation attributes and biological performance.
• Collaborate closely with platform biology, in vivo pharmacology, and process development colleagues to advance candidate tLNP formulations from discovery through early development, and to inform downstream manufacturability considerations.
• Prepare study reports, presentations, and technical documentation summarizing research findings for internal stakeholders.
• Stay current with emerging science in immune cell‑targeted LNP delivery, Treg and lymphocyte biology, and nucleic acid therapeutics, and contribute new ideas to platform strategy.
• Perform other responsibilities as required based on business needs.

Required Qualifications & Experience

• Ph.D. in Immunology or a closely related discipline, with 4+ years of relevant industry or academia experience, with industry or a combination of industry and academia experience preferred. Direct, hands‑on immunology research experience is required.
• Demonstrated hands‑on experience applying targeted lipid nanoparticles (tLNPs) for cell‑specific delivery to immune cells, including design of targeting strategies and evaluation of delivery in primary cells.
• Working familiarity with LNP formulation for mRNA or other nucleic acid payloads sufficient to collaborate effectively with formulation scientists.
• Strong understanding of immunology, with specific experience in lymphocyte biology and in vivo disease models
• Highly organized, scientifically rigorous, and capable of independently driving projects while collaborating effectively in a multidisciplinary startup environment.
• Excellent written and oral communication skills.
• Strong analytical and problem‑solving skills, with great attention to detail and the ability to drive timely, fact‑based decision making.
• Ability to function in a rapidly changing environment and manage multiple priorities.

Preferred Qualifications

• Experience with flow cytometry for cell‑based assay characterization and evaluation of tLNP uptake and targeting.
• Expertise designing and executing in vivo mouse studies, including dosing, biodistribution, and efficacy studies.
• Top‑tier written, verbal, and interpersonal communication skills, with strong organizational skills and attention to detail.
• Prior experience with Treg, CAR‑T, or other engineered cell therapy and immunotherapy platforms.
• Experience correlating formulation or conjugation attributes with in vivo performance.

Other Requirements

• Legally authorized to work for any employer in the U.S.
• Position is onsite in San Diego

The job responsibilities and requirements provided above are intended to describe the general nature of the work performed by individuals assigned to this job classification. It is not intended to be an exhaustive list of all duties and requirements. Tr1X retains the right to add, change, or delete duties, education, experience or any other requirements of the position at any time.

Working at Tr1X

At Tr1X, our team is united by a singular shared mission: to transform patients' lives through breakthrough science. We are a group of skilled experts driven by urgency, innovation and a collaborative spirit. Our culture thrives on excellence, positivity, persistence and the agility to tackle new challenges head‑on. With an unwavering commitment to patients, science and one another, we foster an environment where team members feel engaged, supported and empowered to make a meaningful impact. We envision a world where autoimmune diseases are not just being treated but are cured.

Base Pay Range Anticipated: $152,000 - $165,000

Tr1X considers a variety of factors to